Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8486 and 8487 are separated by 1227 nucleotides8487 and 8488 are separated by 53 nucleotides8488 and 8489 are separated by 155 nucleotides8489 and 8490 are separated by 316 nucleotides mRNA_118: 8486 - HMMPfam-Protein of unknown function (DUF1674)-PF07896, at 278,432 to 278,981 8486 mRNA_119: 8487 - KOG3595 Dyneins, heavy chain, at 280,209 to 281,206 8487 mRNA_120: 8488 - K10413 DYNC1H dynein heavy chain 1, cytosolic, at 281,260 to 295,239 8488 mRNA_121: 8489 - BLAST Pc20g03530 [Penicillium rubens Wisconsin 54-1255], at 295,395 to 296,134 8489 mRNA_122: 8490 - HMMPfam-Rare lipoprotein A (RlpA)-like double-psi beta-barrel-PF03330,SUPERFAMILY--SSF50685, at 296,451 to 297,684 8490
Group Condition mRNA_118 mRNA_119 mRNA_120 mRNA_121 mRNA_122
8486 8487 8488 8489 8490
facs on lipid accumulation Sorted High BODIPY Gate -1.1 N.D. N.D. -1.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.9 N.D. N.D. -0.3 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. N.D. -0.5 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -1.2 N.D. N.D. +0.9 -0.8
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. N.D. +0.8 -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.4 N.D. N.D. +1.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.2 N.D. N.D. +1.3 +0.4
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. +0.3 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. N.D. +0.6 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. N.D. +0.9 +0.1
growth on ypd YPD +1.3 N.D. N.D. +0.2 +0.0
growth on oleic acid YNB Glucose +0.5 N.D. N.D. +0.8 +0.4
auxotrophy YNB Glucose 75mM Arginine +1.5 N.D. N.D. +0.3 +0.4
auxotrophy YNB Glucose +1.2 N.D. N.D. +0.6 +0.7
auxotrophy YNB Glucose 75mM Methionine +1.8 N.D. N.D. +0.1 +0.6
auxotrophy Dropout Complete +1.5 N.D. N.D. +0.4 +0.8
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