Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8495 and 8496 are separated by 388 nucleotides8496 and 8497 are separated by 635 nucleotides8497 and 8498 are separated by 90 nucleotides8498 and 8499 are separated by 186 nucleotides mRNA_127: 8495 - K11229 BCK1 mitogen-activated protein kinase kinase kinase, at 304,252 to 307,207 8495 mRNA_128: 8496 - K11592 DICER1, DCR1 endoribonuclease Dicer, at 307,596 to 312,359 8496 mRNA_129: 8497 - SUPERFAMILY--SSF56112, at 312,995 to 315,210 8497 mRNA_130: 8498 - BLAST 60S ribosomal protein L18-B [Termitomyces sp. J132], at 315,301 to 316,369 8498 mRNA_131: 8499 - K11092 SNRPA1 U2 small nuclear ribonucleoprotein A', at 316,556 to 317,725 8499
Group Condition mRNA_127 mRNA_128 mRNA_129 mRNA_130 mRNA_131
8495 8496 8497 8498 8499
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.0 +0.2 N.D. -1.7
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.1 +0.3 N.D. -1.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.1 +0.1 N.D. -1.3
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.0 -0.3 N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 -0.0 N.D. -0.8
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.1 -0.1 N.D. -0.3
growth on oleic acid YNB Oleic Acid +0.3 +0.2 +0.1 N.D. -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.0 -0.4 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.1 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.1 -0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 -0.1 N.D. -0.2
growth on oleic acid YNB Glucose -0.0 +0.1 +0.3 N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.0 -0.1 N.D. -0.2
auxotrophy YNB Glucose +0.2 +0.1 -0.2 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.0 -0.0 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.2 +0.5 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.1 +0.3 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.0 -0.4 N.D. +0.7
auxotrophy Dropout Complete +0.4 +0.1 -0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.2 -0.0 -0.2 N.D. +0.5
growth on ypd YPD -0.3 +0.1 +0.4 N.D. +1.0
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