Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8506 and 8507 are separated by 323 nucleotides8507 and 8508 are separated by 133 nucleotides8508 and 8509 are separated by 507 nucleotides8509 and 8510 are separated by 915 nucleotides mRNA_138: 8506 - KOG4473 Uncharacterized membrane protein, at 332,077 to 333,617 8506 mRNA_139: 8507 - K14538 NUG1, GNL3 nuclear GTP-binding protein, at 333,941 to 336,511 8507 mRNA_140: 8508 - K14571 RIX7, NVL ribosome biogenesis ATPase, at 336,645 to 339,470 8508 mRNA_141: 8509 - Hypothetical Protein, at 339,978 to 342,089 8509 mRNA_142: 8510 - HMMPfam-Protein of unknown function (DUF3712)-PF12505, at 343,005 to 355,699 8510
Group Condition mRNA_138 mRNA_139 mRNA_140 mRNA_141 mRNA_142
8506 8507 8508 8509 8510
growth on ricinoleic acid YNB Methylricinoleic Acid -1.7 N.D. N.D. -0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -1.2 N.D. N.D. +0.0 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. N.D. -0.4 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. N.D. -0.5 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.7 N.D. N.D. -0.0 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. N.D. -0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 N.D. N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.9 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 N.D. N.D. -0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. -0.4 -0.1
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. N.D. +0.2 -0.0
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.2 -0.0
growth on ricinoleic acid YNB Glucose -0.4 N.D. N.D. +0.4 +0.0
auxotrophy YNB Glucose -0.2 N.D. N.D. +0.2 +0.0
auxotrophy Dropout Complete -0.0 N.D. N.D. +0.4 -0.0
growth on ypd YPD +1.1 N.D. N.D. -0.7 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.2 N.D. N.D. -0.2 +0.0
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