Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8508 and 8509 are separated by 507 nucleotides8509 and 8510 are separated by 915 nucleotides8510 and 8511 are separated by 468 nucleotides8511 and 8512 are separated by 437 nucleotides mRNA_140: 8508 - K14571 RIX7, NVL ribosome biogenesis ATPase, at 336,645 to 339,470 8508 mRNA_141: 8509 - Hypothetical Protein, at 339,978 to 342,089 8509 mRNA_142: 8510 - HMMPfam-Protein of unknown function (DUF3712)-PF12505, at 343,005 to 355,699 8510 mRNA_143: 8511 - KOG4136 Predicted mitochondrial cholesterol transporter, at 356,168 to 357,922 8511 mRNA_144: 8512 - K00698 CHS1 chitin synthase, at 358,360 to 365,387 8512
Group Condition mRNA_140 mRNA_141 mRNA_142 mRNA_143 mRNA_144
8508 8509 8510 8511 8512
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 +0.1 +0.4 -1.5
growth on ypd YPD N.D. -0.7 +0.1 -0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -0.2 -0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 -0.0 +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.0 -0.5 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.3 +0.0 -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.3 -0.1 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 -0.1 +0.4 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.1 +0.1 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 -0.1 +0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.1 -0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.0 -0.0 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 -0.0 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 +0.1 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 -0.0 +0.2 -0.0
auxotrophy YNB Glucose N.D. +0.2 +0.0 -0.1 -0.2
growth on oleic acid YNB Glucose N.D. -0.2 -0.0 +0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 +0.1 +0.2 -0.3
auxotrophy Dropout Complete N.D. +0.4 -0.0 +0.1 -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.4 +0.0 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.0 +0.6 +0.4
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