Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8511 and 8512 are separated by 437 nucleotides8512 and 8513 are separated by 284 nucleotides8513 and 8514 are separated by 128 nucleotides8514 and 8515 are separated by 640 nucleotides mRNA_143: 8511 - KOG4136 Predicted mitochondrial cholesterol transporter, at 356,168 to 357,922 8511 mRNA_144: 8512 - K00698 CHS1 chitin synthase, at 358,360 to 365,387 8512 mRNA_145: 8513 - KOG3299 Uncharacterized conserved protein, at 365,672 to 367,146 8513 mRNA_146: 8514 - HMMPfam-PB1 domain-PF00564,SUPERFAMILY--SSF54277, at 367,275 to 368,042 8514 mRNA_147: 8515 - K20120 BIN3 bridging integrator 3, at 368,683 to 371,935 8515
Group Condition mRNA_143 mRNA_144 mRNA_145 mRNA_146 mRNA_147
8511 8512 8513 8514 8515
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -1.5 -0.0 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy -0.5 -0.0 -0.4 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.2 -0.1 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 -0.1 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.8 +0.2 N.D. +0.4
growth on ypd YPD -0.2 -0.2 -0.3 N.D. +0.4
growth on ricinoleic acid YNB Glucose -0.0 -0.2 +0.4 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.0 -0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.2 -0.3 +0.2 N.D. +0.1
auxotrophy YNB Glucose -0.1 -0.2 +0.3 N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.4 +0.5 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.2 +0.1 N.D. +0.6
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.3 +0.2 N.D. +0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.5 +0.8 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.5 +0.5 N.D. +0.5
growth on oleic acid YNB Glucose +0.1 +0.1 +0.3 N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.1 +0.4 N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.1 +0.1 N.D. +0.6
auxotrophy Dropout Complete +0.1 -0.2 +0.6 N.D. +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.1 +0.7 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.4 -0.0 N.D. +0.5
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