Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8512 and 8513 are separated by 284 nucleotides8513 and 8514 are separated by 128 nucleotides8514 and 8515 are separated by 640 nucleotides8515 and 8516 are separated by 205 nucleotides mRNA_144: 8512 - K00698 CHS1 chitin synthase, at 358,360 to 365,387 8512 mRNA_145: 8513 - KOG3299 Uncharacterized conserved protein, at 365,672 to 367,146 8513 mRNA_146: 8514 - HMMPfam-PB1 domain-PF00564,SUPERFAMILY--SSF54277, at 367,275 to 368,042 8514 mRNA_147: 8515 - K20120 BIN3 bridging integrator 3, at 368,683 to 371,935 8515 mRNA_148: 8516 - KOG0254 Predicted transporter (major facilitator superfamily), at 372,141 to 374,463 8516
Group Condition mRNA_144 mRNA_145 mRNA_146 mRNA_147 mRNA_148
8512 8513 8514 8515 8516
growth on ricinoleic acid YNB Ricinoleic Acid -1.5 -0.0 N.D. -0.3 -0.3
growth on ypd YPD -0.2 -0.3 N.D. +0.4 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.4 N.D. -0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 +0.2 N.D. +0.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.1 N.D. -0.0 +0.3
growth on ricinoleic acid YNB Glucose -0.2 +0.4 N.D. -0.1 -0.2
growth on oleic acid YNB Oleic Acid -0.3 +0.2 N.D. +0.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.5 +0.8 N.D. -0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 N.D. +0.0 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.1 N.D. +0.6 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.0 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 +0.5 N.D. +0.5 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.5 N.D. +0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.4 N.D. -0.0 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.0 N.D. +0.5 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.1 N.D. +0.6 +0.2
growth on oleic acid YNB Glucose +0.1 +0.3 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.7 N.D. +0.4 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.2 N.D. +0.5 +0.3
auxotrophy YNB Glucose -0.2 +0.3 N.D. +0.3 +0.5
auxotrophy Dropout Complete -0.2 +0.6 N.D. +0.6 +0.1
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