Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8527 and 8528 are separated by 734 nucleotides8528 and 8529 are separated by 889 nucleotides8529 and 8530 are separated by 512 nucleotides8530 and 8531 are separated by 259 nucleotides mRNA_159: 8527 - Hypothetical Protein, at 394,494 to 395,592 8527 mRNA_160: 8528 - K05349 bglX beta-glucosidase, at 396,327 to 398,883 8528 mRNA_161: 8529 - KOG1727 Microtubule-binding protein (translationally controlled tumor protein), at 399,773 to 400,854 8529 mRNA_162: 8530 - BLAST NADH dehydrogenase [ubiquinone] 1 alpha subcomplex su..., at 401,367 to 403,016 8530 mRNA_163: 8531 - Hypothetical Protein, at 403,276 to 404,295 8531
Group Condition mRNA_159 mRNA_160 mRNA_161 mRNA_162 mRNA_163
8527 8528 8529 8530 8531
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.4 N.D. -0.7 -0.3
auxotrophy Dropout Complete -0.1 -0.5 N.D. -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 N.D. -0.0 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.0 N.D. -0.5 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.1 N.D. -0.1 -0.6
auxotrophy YNB Glucose -0.1 -0.3 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.2 N.D. -0.9 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.2 N.D. -0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.0 N.D. -0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.2 N.D. -0.4 -0.4
growth on oleic acid YNB Glucose -0.1 -0.4 N.D. -0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 N.D. -0.4 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.3 N.D. -0.2 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.1 N.D. -0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 N.D. -0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.2 N.D. +0.1 +0.3
growth on oleic acid YNB Oleic Acid -0.1 +0.0 N.D. -0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.3 N.D. +0.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.4 N.D. +0.8 -0.9
growth on ricinoleic acid YNB Glucose +0.0 -0.1 N.D. +0.4 +0.6
growth on ypd YPD +0.3 +0.1 N.D. +0.7 +0.5
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