Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8532 and 8533 are separated by 411 nucleotides8533 and 8534 are separated by 313 nucleotides8534 and 8535 are separated by 543 nucleotides8535 and 8536 are separated by 451 nucleotides mRNA_164: 8532 - Hypothetical Protein, at 404,595 to 407,143 8532 mRNA_165: 8533 - K12602 WDR61, REC14, SKI8 WD repeat-containing protein 61, at 407,555 to 409,013 8533 mRNA_166: 8534 - K07936 RAN GTP-binding nuclear protein Ran, at 409,327 to 410,564 8534 mRNA_167: 8535 - KOG1471 Phosphatidylinositol transfer protein SEC14 and related proteins, at 411,108 to 413,532 8535 mRNA_168: 8536 - K01598 PPCDC, coaC phosphopantothenoylcysteine decarboxylase, at 413,984 to 414,971 8536
Group Condition mRNA_164 mRNA_165 mRNA_166 mRNA_167 mRNA_168
8532 8533 8534 8535 8536
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -1.6 N.D. -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.9 N.D. -0.4 N.D.
auxotrophy Dropout Complete -0.0 -0.9 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -1.0 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.8 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.6 N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.5 N.D. -0.0 N.D.
growth on oleic acid YNB Glucose +0.2 -0.9 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.4 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.5 N.D. -0.0 N.D.
auxotrophy YNB Glucose +0.1 -0.5 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.4 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.5 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.2 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.0 -0.6 N.D. +0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.2 -0.1 N.D. -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.0 N.D. +0.1 N.D.
growth on ypd YPD -0.2 +0.4 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.4 N.D. -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 N.D. +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.5 N.D. +0.1 N.D.
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