Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8533 and 8534 are separated by 313 nucleotides8534 and 8535 are separated by 543 nucleotides8535 and 8536 are separated by 451 nucleotides8536 and 8537 are separated by 96 nucleotides mRNA_165: 8533 - K12602 WDR61, REC14, SKI8 WD repeat-containing protein 61, at 407,555 to 409,013 8533 mRNA_166: 8534 - K07936 RAN GTP-binding nuclear protein Ran, at 409,327 to 410,564 8534 mRNA_167: 8535 - KOG1471 Phosphatidylinositol transfer protein SEC14 and related proteins, at 411,108 to 413,532 8535 mRNA_168: 8536 - K01598 PPCDC, coaC phosphopantothenoylcysteine decarboxylase, at 413,984 to 414,971 8536 mRNA_169: 8537 - K14306 NUP62, NSP1 nuclear pore complex protein Nup62, at 415,068 to 417,360 8537
Group Condition mRNA_165 mRNA_166 mRNA_167 mRNA_168 mRNA_169
8533 8534 8535 8536 8537
facs on lipid accumulation Sorted No BODIPY Gate -1.6 N.D. -0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. -0.4 N.D. N.D.
auxotrophy Dropout Complete -0.9 N.D. -0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -1.0 N.D. -0.2 N.D. N.D.
growth on oleic acid YNB Glucose -0.9 N.D. +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.8 N.D. -0.0 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 N.D. +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. -0.0 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.0 N.D. N.D.
auxotrophy YNB Glucose -0.5 N.D. -0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.3 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 N.D. +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.6 N.D. +0.3 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.0 N.D. N.D.
growth on ypd YPD +0.4 N.D. +0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. +0.1 N.D. N.D.
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