Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8536 and 8537 are separated by 96 nucleotides8537 and 8538 are separated by 243 nucleotides8538 and 8539 are separated by 161 nucleotides8539 and 8540 are separated by 499 nucleotides mRNA_168: 8536 - K01598 PPCDC, coaC phosphopantothenoylcysteine decarboxylase, at 413,984 to 414,971 8536 mRNA_169: 8537 - K14306 NUP62, NSP1 nuclear pore complex protein Nup62, at 415,068 to 417,360 8537 mRNA_170: 8538 - K12179 COPS6, CSN6 COP9 signalosome complex subunit 6, at 417,604 to 419,070 8538 mRNA_171: 8539 - K03237 EIF2S1 translation initiation factor 2 subunit 1, at 419,232 to 420,600 8539 mRNA_172: 8540 - K14264 BNA3 kynurenine aminotransferase, at 421,100 to 422,861 8540
Group Condition mRNA_168 mRNA_169 mRNA_170 mRNA_171 mRNA_172
8536 8537 8538 8539 8540
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -1.0 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -1.3 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -1.2 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -1.0 N.D. -0.0
auxotrophy Dropout Complete N.D. N.D. -0.5 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.5 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.5 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.5 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.4 N.D. -0.1
auxotrophy YNB Glucose N.D. N.D. -0.4 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.5 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.0 N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. -0.0
growth on ypd YPD N.D. N.D. +0.1 N.D. +0.0
growth on oleic acid YNB Glucose N.D. N.D. +0.2 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.2 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.3 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.3 N.D. +0.2
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