Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8538 and 8539 are separated by 161 nucleotides8539 and 8540 are separated by 499 nucleotides8540 and 8541 are separated by 381 nucleotides8541 and 8542 are separated by 456 nucleotides mRNA_170: 8538 - K12179 COPS6, CSN6 COP9 signalosome complex subunit 6, at 417,604 to 419,070 8538 mRNA_171: 8539 - K03237 EIF2S1 translation initiation factor 2 subunit 1, at 419,232 to 420,600 8539 mRNA_172: 8540 - K14264 BNA3 kynurenine aminotransferase, at 421,100 to 422,861 8540 mRNA_173: 8541 - ProSiteProfiles-F-box domain profile.-PS50181, at 423,243 to 425,969 8541 mRNA_174: 8542 - HMMPfam-Glyoxal oxidase N-terminus-PF07250,HMMPfam-Domain of unknown function (DUF1929)-PF09118,SUPERFAMILY--SSF50965,SUPERFAMILY--SSF81296, at 426,426 to 428,792 8542
Group Condition mRNA_170 mRNA_171 mRNA_172 mRNA_173 mRNA_174
8538 8539 8540 8541 8542
facs on lipid accumulation Sorted No BODIPY Gate -1.0 N.D. -0.0 +0.2 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. -0.1 -0.2 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -1.3 N.D. +0.1 +0.4 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -1.0 N.D. -0.3 +0.4 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. -0.1 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. -0.0 -0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -1.2 N.D. +0.2 +0.3 +0.1
auxotrophy Dropout Complete -0.5 N.D. -0.4 +0.4 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. -0.2 +0.4 -0.1
growth on ricinoleic acid YNB Glucose -0.5 N.D. -0.2 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. -0.1 -0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.5 +0.1 +0.2
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.2 +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.0 -0.1 +0.2
growth on ypd YPD +0.1 N.D. +0.0 +0.5 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. +0.2 +0.2 -0.4
growth on oleic acid YNB Glucose +0.2 N.D. -0.0 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.2 +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. -0.1 +0.6 +0.3
auxotrophy YNB Glucose -0.4 N.D. -0.1 +0.4 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.2 +0.0 -0.1
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