Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8548 and 8549 are separated by 595 nucleotides8549 and 8550 are separated by 99 nucleotides8550 and 8551 are separated by 207 nucleotides8551 and 8552 are separated by 126 nucleotides mRNA_180: 8548 - K01647 CS, gltA citrate synthase, at 438,943 to 441,127 8548 mRNA_181: 8549 - K12870 ISY1 pre-mRNA-splicing factor ISY1, at 441,723 to 442,875 8549 mRNA_182: 8550 - K19828 MTG1 mitochondrial GTPase 1, at 442,975 to 444,845 8550 mRNA_183: 8551 - KOG4463 Uncharacterized conserved protein, at 445,053 to 446,538 8551 mRNA_184: 8552 - KOG1286 Amino acid transporters, at 446,665 to 448,836 8552
Group Condition mRNA_180 mRNA_181 mRNA_182 mRNA_183 mRNA_184
8548 8549 8550 8551 8552
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. -2.1 -0.5 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. -1.0 +0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 N.D. -0.0 -0.4 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 N.D. -0.3 +0.0 -0.2
growth on ypd YPD -0.2 N.D. -0.4 -0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.4 -1.3 +0.0
growth on oleic acid YNB Oleic Acid -0.0 N.D. -0.0 -0.5 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. +0.1 +0.4 -0.1
growth on ricinoleic acid YNB Glucose -0.4 N.D. +0.3 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. +0.7 -0.4 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.2 -0.3 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.3 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +1.1 -0.6 -0.3
growth on oleic acid YNB Glucose -0.4 N.D. +0.9 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 -0.1 +0.2
auxotrophy Dropout Complete -0.3 N.D. +1.0 -0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. +0.6 +0.6 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. +0.8 +0.2 +0.3
auxotrophy YNB Glucose -0.3 N.D. +1.4 -0.4 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.6 N.D. +1.4 -0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. +1.8 -0.3 -0.1
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