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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8548 and 8549 are separated by 595 nucleotides
8549 and 8550 are separated by 99 nucleotides
8550 and 8551 are separated by 207 nucleotides
8551 and 8552 are separated by 126 nucleotides
mRNA_180: 8548 - K01647 CS, gltA citrate synthase, at 438,943 to 441,127
8548
mRNA_181: 8549 - K12870 ISY1 pre-mRNA-splicing factor ISY1, at 441,723 to 442,875
8549
mRNA_182: 8550 - K19828 MTG1 mitochondrial GTPase 1, at 442,975 to 444,845
8550
mRNA_183: 8551 - KOG4463 Uncharacterized conserved protein, at 445,053 to 446,538
8551
mRNA_184: 8552 - KOG1286 Amino acid transporters, at 446,665 to 448,836
8552
Group
Condition
mRNA
_180
mRNA
_181
mRNA
_182
mRNA
_183
mRNA
_184
8548
8549
8550
8551
8552
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.5
N.D.
-2.1
-0.5
+0.1
facs on lipid accumulation
Sorted High BODIPY Gate
-0.8
N.D.
-1.0
+0.0
+0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.6
N.D.
-0.0
-0.4
-0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.7
N.D.
-0.3
+0.0
-0.2
growth on ypd
YPD
-0.2
N.D.
-0.4
-0.2
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.1
N.D.
+0.4
-1.3
+0.0
growth on oleic acid
YNB Oleic Acid
-0.0
N.D.
-0.0
-0.5
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.8
N.D.
+0.1
+0.4
-0.1
growth on ricinoleic acid
YNB Glucose
-0.4
N.D.
+0.3
-0.0
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.4
N.D.
+0.7
-0.4
-0.0
ypd buoyancy
40 hrs Low Buoyancy
-0.0
N.D.
+0.2
-0.3
+0.0
ypd buoyancy
40 hrs High Buoyancy
+0.1
N.D.
+0.3
-0.1
-0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
N.D.
+1.1
-0.6
-0.3
growth on oleic acid
YNB Glucose
-0.4
N.D.
+0.9
-0.1
-0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.1
N.D.
+0.2
-0.1
+0.2
auxotrophy
Dropout Complete
-0.3
N.D.
+1.0
-0.1
+0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.5
N.D.
+0.6
+0.6
+0.0
facs on lipid accumulation
Sorted No BODIPY Gate
-0.5
N.D.
+0.8
+0.2
+0.3
auxotrophy
YNB Glucose
-0.3
N.D.
+1.4
-0.4
+0.1
auxotrophy
YNB Glucose 75mM Methionine
-0.6
N.D.
+1.4
-0.0
+0.2
auxotrophy
YNB Glucose 75mM Arginine
-0.3
N.D.
+1.8
-0.3
-0.1
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