Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8575 and 8576 are separated by 405 nucleotides8576 and 8577 are separated by 79 nucleotides8577 and 8578 are separated by 436 nucleotides8578 and 8579 are separated by 357 nucleotides mRNA_207: 8575 - K01892 HARS, hisS histidyl-tRNA synthetase, at 506,523 to 508,733 8575 mRNA_208: 8576 - K19270 yfbT, yniC sugar-phosphatase, at 509,139 to 510,075 8576 mRNA_209: 8577 - K12571 PAN2 PAB-dependent poly(A)-specific ribonuclease subunit 2, at 510,155 to 514,679 8577 mRNA_210: 8578 - K08675 PRSS15, PIM1 Lon-like ATP-dependent protease, at 515,116 to 519,191 8578 mRNA_211: 8579 - K00432 gpx glutathione peroxidase, at 519,549 to 520,466 8579
Group Condition mRNA_207 mRNA_208 mRNA_209 mRNA_210 mRNA_211
8575 8576 8577 8578 8579
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.6 -0.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.4 -0.3 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 -0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.4 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.8 +0.6 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 +0.4 N.D. N.D.
auxotrophy YNB Glucose N.D. +0.1 +0.1 N.D. N.D.
growth on ypd YPD N.D. +0.2 +0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 -0.4 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.1 +0.5 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -0.1 +0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.6 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 +0.6 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.3 +1.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 +0.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.8 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 +1.8 N.D. N.D.
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