Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8576 and 8577 are separated by 79 nucleotides8577 and 8578 are separated by 436 nucleotides8578 and 8579 are separated by 357 nucleotides8579 and 8580 are separated by 645 nucleotides mRNA_208: 8576 - K19270 yfbT, yniC sugar-phosphatase, at 509,139 to 510,075 8576 mRNA_209: 8577 - K12571 PAN2 PAB-dependent poly(A)-specific ribonuclease subunit 2, at 510,155 to 514,679 8577 mRNA_210: 8578 - K08675 PRSS15, PIM1 Lon-like ATP-dependent protease, at 515,116 to 519,191 8578 mRNA_211: 8579 - K00432 gpx glutathione peroxidase, at 519,549 to 520,466 8579 mRNA_212: 8580 - K01194 TREH, treA, treF alpha,alpha-trehalase, at 521,112 to 523,919 8580
Group Condition mRNA_208 mRNA_209 mRNA_210 mRNA_211 mRNA_212
8576 8577 8578 8579 8580
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.3 N.D. N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.2 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.6 -0.2 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 -0.4 N.D. N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 +0.6 N.D. N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.4 N.D. N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.0 N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.2 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.2 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.4 N.D. N.D. +0.0
auxotrophy YNB Glucose +0.1 +0.1 N.D. N.D. +0.1
growth on oleic acid YNB Glucose -0.1 +0.6 N.D. N.D. -0.1
auxotrophy Dropout Complete -0.1 +0.5 N.D. N.D. -0.0
growth on ypd YPD +0.2 +0.1 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 +0.2 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +0.1 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.6 N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.3 +1.1 N.D. N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +0.8 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 +0.3 N.D. N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +1.8 N.D. N.D. -0.1
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