Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8585 and 8586 are separated by 38 nucleotides8586 and 8587 are separated by 398 nucleotides8587 and 8588 are separated by 330 nucleotides8588 and 8589 are separated by 482 nucleotides mRNA_217: 8585 - K02292 crtO beta-carotene ketolase (CrtO type), at 538,550 to 540,960 8585 mRNA_218: 8586 - Hypothetical Protein, at 540,999 to 541,232 8586 mRNA_219: 8587 - Hypothetical Protein, at 541,631 to 542,423 8587 mRNA_220: 8588 - K11159 K11159 carotenoid cleavage dioxygenase, at 542,754 to 544,832 8588 mRNA_221: 8589 - HMMPfam-Squalene/phytoene synthase-PF00494,ProSitePatterns-Squalene and phytoene synthases signature 2.-PS01045,SUPERFAMILY--SSF48576,TIGRFAM-CarR_dom_SF lycopene cyclase domain-TIGR03462, at 545,315 to 547,571 8589
Group Condition mRNA_217 mRNA_218 mRNA_219 mRNA_220 mRNA_221
8585 8586 8587 8588 8589
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.5 -0.4 -0.2 -1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.1 -1.3 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.1 -1.1 -0.0 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.2 -0.5 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.2 -1.0 +0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.2 -0.3 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.3 -1.1 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.0 -0.2 -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.1 -0.4 -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.1 -0.5 -0.1 -0.1
growth on oleic acid YNB Glucose +0.1 -0.2 -0.0 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.1 +0.2 +0.3 -0.1
growth on ricinoleic acid YNB Glucose +0.2 +0.1 +0.3 -0.0 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 -0.1 +0.1 -0.0
auxotrophy Dropout Complete -0.5 +0.1 +0.5 +0.3 -0.2
growth on oleic acid YNB Oleic Acid +0.1 -0.1 -0.2 +0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 +0.1 +0.1 +0.2
auxotrophy YNB Glucose +0.0 +0.2 -0.0 +0.2 -0.1
growth on ypd YPD -0.5 +0.4 +0.7 -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.2 +0.3 -0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.8 -0.1 -0.1 +0.1 +1.1
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