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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8585 and 8586 are separated by 38 nucleotides
8586 and 8587 are separated by 398 nucleotides
8587 and 8588 are separated by 330 nucleotides
8588 and 8589 are separated by 482 nucleotides
mRNA_217: 8585 - K02292 crtO beta-carotene ketolase (CrtO type), at 538,550 to 540,960
8585
mRNA_218: 8586 - Hypothetical Protein, at 540,999 to 541,232
8586
mRNA_219: 8587 - Hypothetical Protein, at 541,631 to 542,423
8587
mRNA_220: 8588 - K11159 K11159 carotenoid cleavage dioxygenase, at 542,754 to 544,832
8588
mRNA_221: 8589 - HMMPfam-Squalene/phytoene synthase-PF00494,ProSitePatterns-Squalene and phytoene synthases signature 2.-PS01045,SUPERFAMILY--SSF48576,TIGRFAM-CarR_dom_SF lycopene cyclase domain-TIGR03462, at 545,315 to 547,571
8589
Group
Condition
mRNA
_217
mRNA
_218
mRNA
_219
mRNA
_220
mRNA
_221
8585
8586
8587
8588
8589
facs on lipid accumulation
Sorted Low BODIPY Gate
-1.0
-0.5
-0.4
-0.2
-1.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.4
+0.1
-1.3
-0.4
-0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
+0.1
-1.1
-0.0
-0.2
ypd buoyancy
40 hrs No Buoyancy Separation
+0.2
-0.2
-0.5
-0.3
-0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.4
+0.2
-1.0
+0.1
+0.1
auxotrophy
YNB Glucose 75mM Methionine
-0.4
+0.2
-0.3
+0.0
-0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.0
+0.3
-1.1
-0.1
+0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.2
+0.0
-0.2
-0.2
-0.1
ypd buoyancy
40 hrs Low Buoyancy
-0.2
+0.1
-0.4
-0.0
+0.0
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
+0.1
-0.5
-0.1
-0.1
growth on oleic acid
YNB Glucose
+0.1
-0.2
-0.0
+0.0
-0.0
auxotrophy
YNB Glucose 75mM Arginine
-0.4
-0.1
+0.2
+0.3
-0.1
growth on ricinoleic acid
YNB Glucose
+0.2
+0.1
+0.3
-0.0
-0.5
ypd buoyancy
40 hrs High Buoyancy
+0.2
-0.1
-0.1
+0.1
-0.0
auxotrophy
Dropout Complete
-0.5
+0.1
+0.5
+0.3
-0.2
growth on oleic acid
YNB Oleic Acid
+0.1
-0.1
-0.2
+0.2
+0.2
ypd buoyancy
40 hrs Median Buoyancy
-0.1
-0.0
+0.1
+0.1
+0.2
auxotrophy
YNB Glucose
+0.0
+0.2
-0.0
+0.2
-0.1
growth on ypd
YPD
-0.5
+0.4
+0.7
-0.3
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
+0.2
+0.3
-0.0
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.8
-0.1
-0.1
+0.1
+1.1
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