Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8587 and 8588 are separated by 330 nucleotides8588 and 8589 are separated by 482 nucleotides8589 and 8590 are separated by 215 nucleotides8590 and 8591 are separated by 210 nucleotides mRNA_219: 8587 - Hypothetical Protein, at 541,631 to 542,423 8587 mRNA_220: 8588 - K11159 K11159 carotenoid cleavage dioxygenase, at 542,754 to 544,832 8588 mRNA_221: 8589 - HMMPfam-Squalene/phytoene synthase-PF00494,ProSitePatterns-Squalene and phytoene synthases signature 2.-PS01045,SUPERFAMILY--SSF48576,TIGRFAM-CarR_dom_SF lycopene cyclase domain-TIGR03462, at 545,315 to 547,571 8589 mRNA_222: 8590 - K12795 SUGT1, SGT1 suppressor of G2 allele of SKP1, at 547,787 to 548,767 8590 mRNA_223: 8591 - HMMPfam-Protein of unknown function (DUF1212)-PF06738,HMMPfam-Protein of unknown function (DUF3815)-PF12821, at 548,978 to 553,115 8591
Group Condition mRNA_219 mRNA_220 mRNA_221 mRNA_222 mRNA_223
8587 8588 8589 8590 8591
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.2 -1.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.3 -0.4 -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.1 -0.0 -0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.1 -0.1 +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.0 +0.1 +0.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.3 -0.3 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.0 -0.3 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.1 -0.1 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.0 +0.0 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.2 -0.1 N.D. +0.3
growth on ricinoleic acid YNB Glucose +0.3 -0.0 -0.5 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.0 -0.1 N.D. -0.2
growth on oleic acid YNB Glucose -0.0 +0.0 -0.0 N.D. -0.1
auxotrophy YNB Glucose -0.0 +0.2 -0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.3 -0.1 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 -0.0 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +0.2 N.D. -0.0
auxotrophy Dropout Complete +0.5 +0.3 -0.2 N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.2 +0.2 +0.2 N.D. +0.4
growth on ypd YPD +0.7 -0.3 +0.1 N.D. +0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.1 +1.1 N.D. +0.3
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