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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8587 and 8588 are separated by 330 nucleotides
8588 and 8589 are separated by 482 nucleotides
8589 and 8590 are separated by 215 nucleotides
8590 and 8591 are separated by 210 nucleotides
mRNA_219: 8587 - Hypothetical Protein, at 541,631 to 542,423
8587
mRNA_220: 8588 - K11159 K11159 carotenoid cleavage dioxygenase, at 542,754 to 544,832
8588
mRNA_221: 8589 - HMMPfam-Squalene/phytoene synthase-PF00494,ProSitePatterns-Squalene and phytoene synthases signature 2.-PS01045,SUPERFAMILY--SSF48576,TIGRFAM-CarR_dom_SF lycopene cyclase domain-TIGR03462, at 545,315 to 547,571
8589
mRNA_222: 8590 - K12795 SUGT1, SGT1 suppressor of G2 allele of SKP1, at 547,787 to 548,767
8590
mRNA_223: 8591 - HMMPfam-Protein of unknown function (DUF1212)-PF06738,HMMPfam-Protein of unknown function (DUF3815)-PF12821, at 548,978 to 553,115
8591
Group
Condition
mRNA
_219
mRNA
_220
mRNA
_221
mRNA
_222
mRNA
_223
8587
8588
8589
8590
8591
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.4
-0.2
-1.2
N.D.
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-1.3
-0.4
-0.2
N.D.
+0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-1.1
-0.0
-0.2
N.D.
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-1.1
-0.1
+0.1
N.D.
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
-1.0
+0.1
+0.1
N.D.
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.5
-0.3
-0.3
N.D.
+0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.3
+0.0
-0.3
N.D.
-0.2
facs on lipid accumulation
Sorted No BODIPY Gate
-0.5
-0.1
-0.1
N.D.
+0.0
ypd buoyancy
40 hrs Low Buoyancy
-0.4
-0.0
+0.0
N.D.
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.2
-0.2
-0.1
N.D.
+0.3
growth on ricinoleic acid
YNB Glucose
+0.3
-0.0
-0.5
N.D.
-0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
-0.0
-0.1
N.D.
-0.2
growth on oleic acid
YNB Glucose
-0.0
+0.0
-0.0
N.D.
-0.1
auxotrophy
YNB Glucose
-0.0
+0.2
-0.1
N.D.
-0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.2
+0.3
-0.1
N.D.
-0.3
ypd buoyancy
40 hrs High Buoyancy
-0.1
+0.1
-0.0
N.D.
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.1
+0.1
+0.2
N.D.
-0.0
auxotrophy
Dropout Complete
+0.5
+0.3
-0.2
N.D.
-0.2
growth on oleic acid
YNB Oleic Acid
-0.2
+0.2
+0.2
N.D.
+0.4
growth on ypd
YPD
+0.7
-0.3
+0.1
N.D.
+0.5
facs on lipid accumulation
Sorted High BODIPY Gate
-0.1
+0.1
+1.1
N.D.
+0.3
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