Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8589 and 8590 are separated by 215 nucleotides8590 and 8591 are separated by 210 nucleotides8591 and 8592 are separated by 1119 nucleotides8592 and 8593 are separated by 423 nucleotides mRNA_221: 8589 - HMMPfam-Squalene/phytoene synthase-PF00494,ProSitePatterns-Squalene and phytoene synthases signature 2.-PS01045,SUPERFAMILY--SSF48576,TIGRFAM-CarR_dom_SF lycopene cyclase domain-TIGR03462, at 545,315 to 547,571 8589 mRNA_222: 8590 - K12795 SUGT1, SGT1 suppressor of G2 allele of SKP1, at 547,787 to 548,767 8590 mRNA_223: 8591 - HMMPfam-Protein of unknown function (DUF1212)-PF06738,HMMPfam-Protein of unknown function (DUF3815)-PF12821, at 548,978 to 553,115 8591 mRNA_224: 8592 - K07300 chaA, CAX Ca2+-H+ antiporter, at 554,235 to 556,538 8592 mRNA_225: 8593 - KOG0619 FOG Leucine rich repeat, at 556,962 to 559,070 8593
Group Condition mRNA_221 mRNA_222 mRNA_223 mRNA_224 mRNA_225
8589 8590 8591 8592 8593
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.3 -1.8 -0.2
growth on ricinoleic acid YNB Glucose -0.5 N.D. -0.0 -1.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.3 -1.3 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.2 -1.2 +0.0
auxotrophy Dropout Complete -0.2 N.D. -0.2 -0.9 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. -0.2 -0.7 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.0 -0.3 -0.7
auxotrophy YNB Glucose -0.1 N.D. -0.1 -0.7 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.0 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.1 -0.4 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.2 -0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. +0.3 -1.3 +0.7
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.1 -0.8 +0.3
growth on oleic acid YNB Glucose -0.0 N.D. -0.1 +0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.2 N.D. -0.1 +2.3 -0.9
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.4 -0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.0 -0.1 +0.6
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.1 +0.3 +0.5
growth on ypd YPD +0.1 N.D. +0.5 +0.6 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.0 +2.4 -0.5
facs on lipid accumulation Sorted High BODIPY Gate +1.1 N.D. +0.3 +3.4 -0.2
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