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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8589 and 8590 are separated by 215 nucleotides
8590 and 8591 are separated by 210 nucleotides
8591 and 8592 are separated by 1119 nucleotides
8592 and 8593 are separated by 423 nucleotides
mRNA_221: 8589 - HMMPfam-Squalene/phytoene synthase-PF00494,ProSitePatterns-Squalene and phytoene synthases signature 2.-PS01045,SUPERFAMILY--SSF48576,TIGRFAM-CarR_dom_SF lycopene cyclase domain-TIGR03462, at 545,315 to 547,571
8589
mRNA_222: 8590 - K12795 SUGT1, SGT1 suppressor of G2 allele of SKP1, at 547,787 to 548,767
8590
mRNA_223: 8591 - HMMPfam-Protein of unknown function (DUF1212)-PF06738,HMMPfam-Protein of unknown function (DUF3815)-PF12821, at 548,978 to 553,115
8591
mRNA_224: 8592 - K07300 chaA, CAX Ca2+-H+ antiporter, at 554,235 to 556,538
8592
mRNA_225: 8593 - KOG0619 FOG Leucine rich repeat, at 556,962 to 559,070
8593
Group
Condition
mRNA
_221
mRNA
_222
mRNA
_223
mRNA
_224
mRNA
_225
8589
8590
8591
8592
8593
auxotrophy
YNB Glucose 75mM Arginine
-0.1
N.D.
-0.3
-1.8
-0.2
growth on ricinoleic acid
YNB Glucose
-0.5
N.D.
-0.0
-1.2
-0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
N.D.
+0.3
-1.3
-0.5
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
N.D.
-0.2
-1.2
+0.0
auxotrophy
Dropout Complete
-0.2
N.D.
-0.2
-0.9
-0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.3
N.D.
-0.2
-0.7
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
N.D.
+0.0
-0.3
-0.7
auxotrophy
YNB Glucose
-0.1
N.D.
-0.1
-0.7
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
N.D.
-0.0
-0.4
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
N.D.
-0.1
-0.4
-0.5
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
N.D.
-0.2
-0.2
-0.3
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
N.D.
+0.3
-1.3
+0.7
ypd buoyancy
40 hrs Low Buoyancy
+0.0
N.D.
-0.1
-0.8
+0.3
growth on oleic acid
YNB Glucose
-0.0
N.D.
-0.1
+0.2
-0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
-1.2
N.D.
-0.1
+2.3
-0.9
growth on oleic acid
YNB Oleic Acid
+0.2
N.D.
+0.4
-0.1
-0.3
ypd buoyancy
40 hrs Median Buoyancy
+0.2
N.D.
-0.0
-0.1
+0.6
ypd buoyancy
40 hrs High Buoyancy
-0.0
N.D.
+0.1
+0.3
+0.5
growth on ypd
YPD
+0.1
N.D.
+0.5
+0.6
+0.2
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
N.D.
+0.0
+2.4
-0.5
facs on lipid accumulation
Sorted High BODIPY Gate
+1.1
N.D.
+0.3
+3.4
-0.2
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