Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8620 and 8621 are separated by 159 nucleotides8621 and 8622 are separated by 292 nucleotides8622 and 8623 are separated by 297 nucleotides8623 and 8624 are separated by 273 nucleotides mRNA_252: 8620 - Hypothetical Protein, at 609,608 to 610,870 8620 mRNA_253: 8621 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 611,030 to 612,229 8621 mRNA_254: 8622 - KOG0970 DNA polymerase alpha, catalytic subunit, at 612,522 to 613,328 8622 mRNA_255: 8623 - K02320 POLA1 DNA polymerase alpha subunit A, at 613,626 to 618,274 8623 mRNA_256: 8624 - K11254 H4 histone H4, at 618,548 to 619,064 8624
Group Condition mRNA_252 mRNA_253 mRNA_254 mRNA_255 mRNA_256
8620 8621 8622 8623 8624
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.5 N.D. N.D. -1.7
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.3 N.D. N.D. -1.1
auxotrophy YNB Glucose -0.1 -0.6 N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.6 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.4 N.D. N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.3 N.D. N.D. -0.7
auxotrophy Dropout Complete +0.2 -0.6 N.D. N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.2 N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.4 N.D. N.D. +0.3
growth on oleic acid YNB Glucose -0.3 +0.0 N.D. N.D. +0.1
growth on ypd YPD -0.2 +0.2 N.D. N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.0 +0.1 N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.0 N.D. N.D. +0.7
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.4 N.D. N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.0 N.D. N.D. +0.7
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.0 N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.5 N.D. N.D. +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.1 N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.1 N.D. N.D. +0.9
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