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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8620 and 8621 are separated by 159 nucleotides
8621 and 8622 are separated by 292 nucleotides
8622 and 8623 are separated by 297 nucleotides
8623 and 8624 are separated by 273 nucleotides
mRNA_252: 8620 - Hypothetical Protein, at 609,608 to 610,870
8620
mRNA_253: 8621 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 611,030 to 612,229
8621
mRNA_254: 8622 - KOG0970 DNA polymerase alpha, catalytic subunit, at 612,522 to 613,328
8622
mRNA_255: 8623 - K02320 POLA1 DNA polymerase alpha subunit A, at 613,626 to 618,274
8623
mRNA_256: 8624 - K11254 H4 histone H4, at 618,548 to 619,064
8624
Group
Condition
mRNA
_252
mRNA
_253
mRNA
_254
mRNA
_255
mRNA
_256
8620
8621
8622
8623
8624
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
+0.5
N.D.
N.D.
-1.7
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
+0.3
N.D.
N.D.
-1.1
auxotrophy
YNB Glucose
-0.1
-0.6
N.D.
N.D.
-0.0
auxotrophy
YNB Glucose 75mM Arginine
-0.0
-0.6
N.D.
N.D.
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
-0.4
N.D.
N.D.
-0.4
growth on oleic acid
YNB Oleic Acid
+0.1
-0.1
N.D.
N.D.
-0.4
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
-0.2
N.D.
N.D.
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
+0.3
N.D.
N.D.
-0.7
auxotrophy
Dropout Complete
+0.2
-0.6
N.D.
N.D.
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.0
-0.2
N.D.
N.D.
-0.0
auxotrophy
YNB Glucose 75mM Methionine
-0.1
-0.4
N.D.
N.D.
+0.3
growth on oleic acid
YNB Glucose
-0.3
+0.0
N.D.
N.D.
+0.1
growth on ypd
YPD
-0.2
+0.2
N.D.
N.D.
+0.2
growth on ricinoleic acid
YNB Glucose
+0.0
+0.1
N.D.
N.D.
+0.3
ypd buoyancy
40 hrs Low Buoyancy
-0.2
+0.0
N.D.
N.D.
+0.7
ypd buoyancy
40 hrs Median Buoyancy
-0.2
+0.4
N.D.
N.D.
+0.4
ypd buoyancy
40 hrs High Buoyancy
-0.0
+0.0
N.D.
N.D.
+0.7
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
+0.0
N.D.
N.D.
+0.7
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.4
-0.5
N.D.
N.D.
+0.9
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.4
+0.1
N.D.
N.D.
+0.6
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.4
-0.1
N.D.
N.D.
+0.9
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