Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8621 and 8622 are separated by 292 nucleotides8622 and 8623 are separated by 297 nucleotides8623 and 8624 are separated by 273 nucleotides8624 and 8625 are separated by 698 nucleotides mRNA_253: 8621 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 611,030 to 612,229 8621 mRNA_254: 8622 - KOG0970 DNA polymerase alpha, catalytic subunit, at 612,522 to 613,328 8622 mRNA_255: 8623 - K02320 POLA1 DNA polymerase alpha subunit A, at 613,626 to 618,274 8623 mRNA_256: 8624 - K11254 H4 histone H4, at 618,548 to 619,064 8624 mRNA_257: 8625 - KOG1745 Histones H3 and H4, at 619,763 to 620,480 8625
Group Condition mRNA_253 mRNA_254 mRNA_255 mRNA_256 mRNA_257
8621 8622 8623 8624 8625
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. N.D. -1.7 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. N.D. -1.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 N.D. N.D. -0.4 N.D.
auxotrophy YNB Glucose -0.6 N.D. N.D. -0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. -0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.6 N.D. N.D. +0.0 N.D.
auxotrophy Dropout Complete -0.6 N.D. N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. N.D. -0.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. N.D. +0.3 N.D.
growth on oleic acid YNB Glucose +0.0 N.D. N.D. +0.1 N.D.
growth on ypd YPD +0.2 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. N.D. +0.9 N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. +0.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.6 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.7 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.9 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. +0.7 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. N.D. +0.4 N.D.
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