Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8633 and 8634 are separated by 141 nucleotides8634 and 8635 are separated by 345 nucleotides8635 and 8636 are separated by 253 nucleotides8636 and 8637 are separated by 129 nucleotides mRNA_265: 8633 - K00876 udk, UCK uridine kinase, at 637,479 to 639,717 8633 mRNA_266: 8634 - KOG0111 Cyclophilin-type peptidyl-prolyl cis-trans isomerase, at 639,859 to 640,522 8634 mRNA_267: 8635 - K03381 catA catechol 1,2-dioxygenase, at 640,868 to 642,235 8635 mRNA_268: 8636 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 642,489 to 643,919 8636 mRNA_269: 8637 - BLAST nitrogen assimilation transcription factor [Rhodo..., at 644,049 to 645,349 8637
Group Condition mRNA_265 mRNA_266 mRNA_267 mRNA_268 mRNA_269
8633 8634 8635 8636 8637
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -2.7 -0.5 +0.1
growth on oleic acid YNB Glucose +0.5 N.D. -1.9 -0.3 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. -0.6 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. -1.6 +0.7 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. -1.0 +0.2 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. -0.2 -0.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.7 +0.4 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -0.6 -0.0 +0.3
growth on ypd YPD +0.5 N.D. -0.6 -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.4 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.8 -0.1 +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.0 -0.6 +0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.2 -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.5 +0.2 -0.4
auxotrophy YNB Glucose +0.3 N.D. +0.2 +0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. +0.6 +0.1 +0.0
auxotrophy Dropout Complete +0.3 N.D. +0.6 -0.1 -0.1
growth on oleic acid YNB Oleic Acid +0.4 N.D. -0.2 +0.5 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. -0.0 +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. +0.6 -0.4 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. +1.6 -0.7 +0.8
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