Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8635 and 8636 are separated by 253 nucleotides8636 and 8637 are separated by 129 nucleotides8637 and 8638 are separated by 272 nucleotides8638 and 8639 are separated by 317 nucleotides mRNA_267: 8635 - K03381 catA catechol 1,2-dioxygenase, at 640,868 to 642,235 8635 mRNA_268: 8636 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 642,489 to 643,919 8636 mRNA_269: 8637 - BLAST nitrogen assimilation transcription factor [Rhodo..., at 644,049 to 645,349 8637 mRNA_270: 8638 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 645,622 to 646,120 8638 mRNA_271: 8639 - K11262 ACACA acetyl-CoA carboxylase / biotin carboxylase 1, at 646,438 to 653,784 8639
Group Condition mRNA_267 mRNA_268 mRNA_269 mRNA_270 mRNA_271
8635 8636 8637 8638 8639
facs on lipid accumulation Sorted High BODIPY Gate -2.7 -0.5 +0.1 N.D. N.D.
growth on oleic acid YNB Glucose -1.9 -0.3 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 +0.2 -0.3 N.D. N.D.
growth on ypd YPD -0.6 -0.2 -0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.1 -0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 -0.4 +0.2 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 +0.4 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.6 +0.7 +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 -0.0 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 -0.1 +0.5 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.6 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.2 -0.2 -0.1 N.D. N.D.
auxotrophy YNB Glucose +0.2 +0.2 -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.2 -0.4 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.2 +0.5 +0.1 N.D. N.D.
auxotrophy Dropout Complete +0.6 -0.1 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.3 +0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.6 -0.4 +0.4 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 +0.1 +0.0 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +1.6 -0.7 +0.8 N.D. N.D.
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