Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8650 and 8651 are separated by 698 nucleotides8651 and 8652 are separated by 768 nucleotides8652 and 8653 are separated by 339 nucleotides8653 and 8654 are separated by 728 nucleotides mRNA_282: 8650 - Hypothetical Protein, at 672,440 to 673,215 8650 mRNA_283: 8651 - K00872 thrB1 homoserine kinase, at 673,914 to 675,244 8651 mRNA_284: 8652 - K01762 ACS 1-aminocyclopropane-1-carboxylate synthase, at 676,013 to 677,803 8652 mRNA_285: 8653 - Hypothetical Protein, at 678,143 to 678,782 8653 mRNA_286: 8654 - SUPERFAMILY--SSF52047, at 679,511 to 681,745 8654
Group Condition mRNA_282 mRNA_283 mRNA_284 mRNA_285 mRNA_286
8650 8651 8652 8653 8654
auxotrophy Dropout Complete -0.0 N.D. -0.9 -0.7 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. +0.4 -0.4 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.4 -0.1 +0.1
auxotrophy YNB Glucose +0.3 N.D. -0.1 -0.7 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. -0.3 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.3 -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. +0.2 +0.2 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.5 -0.4 +0.6
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. -0.4 -0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. +0.6 +0.2 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.3 -0.2 +0.1
growth on oleic acid YNB Glucose +0.3 N.D. +0.1 +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.0 +0.6 -0.2
growth on ricinoleic acid YNB Glucose +0.0 N.D. -0.4 +0.2 +0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. +1.0 +0.7 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.2 +0.8 +0.4
growth on oleic acid YNB Oleic Acid +0.3 N.D. +0.0 +0.4 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.1 +1.0 -0.1
growth on ypd YPD +0.1 N.D. +0.6 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 N.D. +0.1 +1.2 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.1 +0.9 +0.7
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