Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8652 and 8653 are separated by 339 nucleotides8653 and 8654 are separated by 728 nucleotides8654 and 8655 are separated by 253 nucleotides8655 and 8656 are separated by 194 nucleotides mRNA_284: 8652 - K01762 ACS 1-aminocyclopropane-1-carboxylate synthase, at 676,013 to 677,803 8652 mRNA_285: 8653 - Hypothetical Protein, at 678,143 to 678,782 8653 mRNA_286: 8654 - SUPERFAMILY--SSF52047, at 679,511 to 681,745 8654 mRNA_287: 8655 - KOG1861 Leucine permease transcriptional regulator, at 681,999 to 683,854 8655 mRNA_288: 8656 - K00521 E1.16.1.7 ferric-chelate reductase, at 684,049 to 686,611 8656
Group Condition mRNA_284 mRNA_285 mRNA_286 mRNA_287 mRNA_288
8652 8653 8654 8655 8656
auxotrophy Dropout Complete -0.9 -0.7 +0.2 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.1 +0.1 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.4 -0.1 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 +0.2 N.D. -0.3
auxotrophy YNB Glucose -0.1 -0.7 +0.1 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.2 -0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.2 +0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.2 +0.3 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.4 +0.6 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.2 +0.1 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.6 -0.2 N.D. -0.3
growth on oleic acid YNB Glucose +0.1 +0.2 -0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +1.0 +0.7 -0.5 N.D. -0.9
facs on lipid accumulation Sorted High BODIPY Gate +0.6 +0.2 -0.5 N.D. +0.0
growth on ricinoleic acid YNB Glucose -0.4 +0.2 +0.8 N.D. -0.1
growth on oleic acid YNB Oleic Acid +0.0 +0.4 +0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +1.0 -0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.8 +0.4 N.D. -0.0
growth on ypd YPD +0.6 +0.4 -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.9 +0.7 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +1.2 +0.8 N.D. -0.0
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