Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8657 and 8658 are separated by 358 nucleotides8658 and 8659 are separated by 570 nucleotides8659 and 8660 are separated by 162 nucleotides8660 and 8661 are separated by 541 nucleotides mRNA_289: 8657 - HMMPfam-Major Facilitator Superfamily-PF07690,ProSiteProfiles-Major facilitator superfamily (MFS) profile.-PS50850,SUPERFAMILY--SSF103473, at 686,748 to 688,788 8657 mRNA_290: 8658 - HMMPfam-Major Facilitator Superfamily-PF07690,ProSitePatterns-Sugar transport proteins signature 1.-PS00216,ProSiteProfiles-Major facilitator superfamily (MFS) profile.-PS50850,SUPERFAMILY--SSF103473, at 689,147 to 690,940 8658 mRNA_291: 8659 - K03012 RPB4, POLR2D DNA-directed RNA polymerase II subunit RPB4, at 691,511 to 692,003 8659 mRNA_292: 8660 - HMMPfam-Endoplasmic reticulum-based factor for assembly of V-ATPase-PF11712, at 692,166 to 693,130 8660 mRNA_293: 8661 - Hypothetical Protein, at 693,672 to 694,229 8661
Group Condition mRNA_289 mRNA_290 mRNA_291 mRNA_292 mRNA_293
8657 8658 8659 8660 8661
growth on oleic acid YNB Glucose +0.1 -0.1 N.D. N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.2 N.D. N.D. -0.7
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.5 N.D. N.D. -0.7
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.3 N.D. N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 N.D. N.D. -0.6
growth on oleic acid YNB Oleic Acid -0.1 +0.0 N.D. N.D. -0.2
growth on ricinoleic acid YNB Glucose -0.2 +0.4 N.D. N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.1 N.D. N.D. -0.3
auxotrophy Dropout Complete -0.1 +0.0 N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.2 N.D. N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.4 N.D. N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.0 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.0 N.D. N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.4 N.D. N.D. -0.1
growth on ypd YPD -0.0 +0.1 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.3 N.D. N.D. +0.4
auxotrophy YNB Glucose +0.3 -0.0 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 N.D. N.D. +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.3 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.0 N.D. N.D. +0.6
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