Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8658 and 8659 are separated by 570 nucleotides8659 and 8660 are separated by 162 nucleotides8660 and 8661 are separated by 541 nucleotides8661 and 8662 are separated by 603 nucleotides mRNA_290: 8658 - HMMPfam-Major Facilitator Superfamily-PF07690,ProSitePatterns-Sugar transport proteins signature 1.-PS00216,ProSiteProfiles-Major facilitator superfamily (MFS) profile.-PS50850,SUPERFAMILY--SSF103473, at 689,147 to 690,940 8658 mRNA_291: 8659 - K03012 RPB4, POLR2D DNA-directed RNA polymerase II subunit RPB4, at 691,511 to 692,003 8659 mRNA_292: 8660 - HMMPfam-Endoplasmic reticulum-based factor for assembly of V-ATPase-PF11712, at 692,166 to 693,130 8660 mRNA_293: 8661 - Hypothetical Protein, at 693,672 to 694,229 8661 mRNA_294: 8662 - Hypothetical Protein, at 694,833 to 695,851 8662
Group Condition mRNA_290 mRNA_291 mRNA_292 mRNA_293 mRNA_294
8658 8659 8660 8661 8662
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. N.D. -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. -0.7 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. -0.6 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.5 N.D. N.D. -0.7 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. -0.0 N.D.
growth on oleic acid YNB Oleic Acid +0.0 N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. N.D. -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. N.D. -0.3 N.D.
auxotrophy Dropout Complete +0.0 N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. N.D. -0.4 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. N.D. -0.4 N.D.
auxotrophy YNB Glucose -0.0 N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. N.D. +0.4 N.D.
growth on ypd YPD +0.1 N.D. N.D. +0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. +0.6 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. +0.3 N.D.
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