Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8659 and 8660 are separated by 162 nucleotides8660 and 8661 are separated by 541 nucleotides8661 and 8662 are separated by 603 nucleotides8662 and 8663 are separated by 231 nucleotides mRNA_291: 8659 - K03012 RPB4, POLR2D DNA-directed RNA polymerase II subunit RPB4, at 691,511 to 692,003 8659 mRNA_292: 8660 - HMMPfam-Endoplasmic reticulum-based factor for assembly of V-ATPase-PF11712, at 692,166 to 693,130 8660 mRNA_293: 8661 - Hypothetical Protein, at 693,672 to 694,229 8661 mRNA_294: 8662 - Hypothetical Protein, at 694,833 to 695,851 8662 mRNA_295: 8663 - KOG3069 Peroxisomal NUDIX hydrolase, at 696,083 to 698,097 8663
Group Condition mRNA_291 mRNA_292 mRNA_293 mRNA_294 mRNA_295
8659 8660 8661 8662 8663
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.7 N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.4 N.D. -0.7
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.6 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.7 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.3 N.D. -0.3
auxotrophy Dropout Complete N.D. N.D. -0.1 N.D. -0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.4 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.3 N.D. -0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.5 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.4 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 N.D. -0.1
auxotrophy YNB Glucose N.D. N.D. +0.1 N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.5 N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.6 N.D. -0.4
growth on ypd YPD N.D. N.D. +0.0 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.4 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.3 N.D. -0.0
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