Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8673 and 8674 are separated by 490 nucleotides8674 and 8675 are separated by 343 nucleotides8675 and 8676 are separated by 351 nucleotides8676 and 8677 are separated by 273 nucleotides mRNA_305: 8673 - HMMPfam-Peroxisomal biogenesis factor 11 (PEX11)-PF05648, at 726,265 to 727,388 8673 mRNA_306: 8674 - HMMPfam-Relaxase/Mobilisation nuclease domain-PF03432,ProSiteProfiles-Tudor domain profile.-PS50304,SMART-Tudor domain-SM00333,SUPERFAMILY--SSF63748, at 727,879 to 728,992 8674 mRNA_307: 8675 - K12195 CHMP6, VPS20 charged multivesicular body protein 6, at 729,336 to 730,471 8675 mRNA_308: 8676 - K07952 ARFRP1 ADP-ribosylation factor related protein 1, at 730,823 to 731,905 8676 mRNA_309: 8677 - K00643 E2.3.1.37, ALAS 5-aminolevulinate synthase, at 732,179 to 734,206 8677
Group Condition mRNA_305 mRNA_306 mRNA_307 mRNA_308 mRNA_309
8673 8674 8675 8676 8677
growth on ricinoleic acid YNB Ricinoleic Acid -4.0 N.D. N.D. +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -3.5 N.D. N.D. +0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. N.D. -1.2 N.D.
growth on oleic acid YNB Oleic Acid -1.8 N.D. N.D. +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.5 N.D. N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 N.D. N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. N.D. +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. N.D. +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. -0.3 N.D.
auxotrophy YNB Glucose +0.2 N.D. N.D. -0.1 N.D.
growth on ypd YPD +0.3 N.D. N.D. -0.2 N.D.
growth on oleic acid YNB Glucose +0.2 N.D. N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. +0.1 N.D.
auxotrophy Dropout Complete +0.5 N.D. N.D. +0.1 N.D.
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