Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8674 and 8675 are separated by 343 nucleotides8675 and 8676 are separated by 351 nucleotides8676 and 8677 are separated by 273 nucleotides8677 and 8678 are separated by 220 nucleotides mRNA_306: 8674 - HMMPfam-Relaxase/Mobilisation nuclease domain-PF03432,ProSiteProfiles-Tudor domain profile.-PS50304,SMART-Tudor domain-SM00333,SUPERFAMILY--SSF63748, at 727,879 to 728,992 8674 mRNA_307: 8675 - K12195 CHMP6, VPS20 charged multivesicular body protein 6, at 729,336 to 730,471 8675 mRNA_308: 8676 - K07952 ARFRP1 ADP-ribosylation factor related protein 1, at 730,823 to 731,905 8676 mRNA_309: 8677 - K00643 E2.3.1.37, ALAS 5-aminolevulinate synthase, at 732,179 to 734,206 8677 mRNA_310: 8678 - K00626 E2.3.1.9, atoB acetyl-CoA C-acetyltransferase, at 734,427 to 736,325 8678
Group Condition mRNA_306 mRNA_307 mRNA_308 mRNA_309 mRNA_310
8674 8675 8676 8677 8678
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.2 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 N.D. N.D.
growth on ypd YPD N.D. N.D. -0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 N.D. N.D.
auxotrophy YNB Glucose N.D. N.D. -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.0 N.D. N.D.
growth on oleic acid YNB Glucose N.D. N.D. +0.0 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.0 N.D. N.D.
auxotrophy Dropout Complete N.D. N.D. +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.3 N.D. N.D.
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