Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8676 and 8677 are separated by 273 nucleotides8677 and 8678 are separated by 220 nucleotides8678 and 8679 are separated by 1195 nucleotides8679 and 8680 are separated by 283 nucleotides mRNA_308: 8676 - K07952 ARFRP1 ADP-ribosylation factor related protein 1, at 730,823 to 731,905 8676 mRNA_309: 8677 - K00643 E2.3.1.37, ALAS 5-aminolevulinate synthase, at 732,179 to 734,206 8677 mRNA_310: 8678 - K00626 E2.3.1.9, atoB acetyl-CoA C-acetyltransferase, at 734,427 to 736,325 8678 mRNA_311: 8679 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 737,521 to 741,046 8679 mRNA_312: 8680 - K20103 DDX60 ATP-dependent RNA helicase DDX60, at 741,330 to 747,566 8680
Group Condition mRNA_308 mRNA_309 mRNA_310 mRNA_311 mRNA_312
8676 8677 8678 8679 8680
facs on lipid accumulation Sorted Low BODIPY Gate -1.2 N.D. N.D. +0.3 N.D.
growth on ypd YPD -0.2 N.D. N.D. -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. N.D. -0.2 N.D.
auxotrophy YNB Glucose -0.1 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. -0.2 N.D.
growth on oleic acid YNB Glucose +0.0 N.D. N.D. -0.3 N.D.
auxotrophy Dropout Complete +0.1 N.D. N.D. -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. N.D. -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.2 N.D.
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. +0.3 N.D.
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