Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8677 and 8678 are separated by 220 nucleotides8678 and 8679 are separated by 1195 nucleotides8679 and 8680 are separated by 283 nucleotides8680 and 8681 are separated by 248 nucleotides mRNA_309: 8677 - K00643 E2.3.1.37, ALAS 5-aminolevulinate synthase, at 732,179 to 734,206 8677 mRNA_310: 8678 - K00626 E2.3.1.9, atoB acetyl-CoA C-acetyltransferase, at 734,427 to 736,325 8678 mRNA_311: 8679 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 737,521 to 741,046 8679 mRNA_312: 8680 - K20103 DDX60 ATP-dependent RNA helicase DDX60, at 741,330 to 747,566 8680 mRNA_313: 8681 - Hypothetical Protein, at 747,815 to 749,312 8681
Group Condition mRNA_309 mRNA_310 mRNA_311 mRNA_312 mRNA_313
8677 8678 8679 8680 8681
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 N.D. -3.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.2 N.D. -3.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 N.D. -3.2
growth on oleic acid YNB Glucose N.D. N.D. -0.3 N.D. -2.7
auxotrophy YNB Glucose N.D. N.D. -0.2 N.D. -2.5
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.2 N.D. -2.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 N.D. -1.8
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 N.D. -1.5
growth on ypd YPD N.D. N.D. -0.4 N.D. -1.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.3 N.D. -1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 N.D. -0.7
auxotrophy Dropout Complete N.D. N.D. -0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.0 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.2 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.3 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 N.D. +0.9
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