Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8680 and 8681 are separated by 248 nucleotides8681 and 8682 are separated by 296 nucleotides8682 and 8683 are separated by 224 nucleotides8683 and 8684 are separated by 321 nucleotides mRNA_312: 8680 - K20103 DDX60 ATP-dependent RNA helicase DDX60, at 741,330 to 747,566 8680 mRNA_313: 8681 - Hypothetical Protein, at 747,815 to 749,312 8681 mRNA_314: 8682 - K13091 RBM39, RNPC2 RNA-binding protein 39, at 749,609 to 751,814 8682 mRNA_315: 8683 - Hypothetical Protein, at 752,039 to 753,953 8683 mRNA_316: 8684 - K18802 DUG3 glutamine amidotransferase, at 754,275 to 756,026 8684
Group Condition mRNA_312 mRNA_313 mRNA_314 mRNA_315 mRNA_316
8680 8681 8682 8683 8684
growth on oleic acid YNB Oleic Acid N.D. -3.3 N.D. +0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -3.3 N.D. +0.2 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -3.2 N.D. +0.2 +0.4
growth on oleic acid YNB Glucose N.D. -2.7 N.D. +0.0 +0.4
growth on ricinoleic acid YNB Glucose N.D. -2.1 N.D. +0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -1.5 N.D. -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -1.8 N.D. +0.1 +0.2
auxotrophy YNB Glucose N.D. -2.5 N.D. +0.4 +0.6
growth on ypd YPD N.D. -1.4 N.D. +0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.1 N.D. +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 N.D. -0.3 -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.7 N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 N.D. -0.1 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 N.D. -0.1 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 N.D. -0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 N.D. -0.0 +0.0
auxotrophy Dropout Complete N.D. -0.3 N.D. +0.1 +0.7
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.9 N.D. +0.0 +0.1
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