Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8685 and 8686 are separated by 81 nucleotides8686 and 8687 are separated by 85 nucleotides8687 and 8688 are separated by 118 nucleotides8688 and 8689 are separated by 57 nucleotides mRNA_317: 8685 - HMMPfam-DnaJ domain-PF00226,PRINTS-DnaJ domain signature-PR00625,ProSitePatterns-Nt-dnaJ domain signature.-PS00636,ProSiteProfiles-dnaJ domain profile.-PS50076,ProSiteProfiles-IQ motif profile.-PS50096,SMART-DnaJ molecular chaperone homology domain-SM00271,SUPERFAMILY--SSF46565, at 756,228 to 757,512 8685 mRNA_318: 8686 - Interpro Fungal domain of unknown function (DUF1750), at 757,594 to 759,249 8686 mRNA_319: 8687 - KOG2134 Polynucleotide kinase 3' phosphatase, at 759,335 to 760,537 8687 mRNA_320: 8688 - Hypothetical Protein, at 760,656 to 762,781 8688 mRNA_321: 8689 - KOG4317 Predicted Zn-finger protein, at 762,839 to 764,616 8689
Group Condition mRNA_317 mRNA_318 mRNA_319 mRNA_320 mRNA_321
8685 8686 8687 8688 8689
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -1.5 -0.5 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -1.3 +0.2 -0.6 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -1.8 +0.2 -0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.9 -0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -1.3 +0.4 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -1.3 +0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.8 +0.6 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.6 +0.6 -0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.3 -0.6 -0.6 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 -0.3 +0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 -0.3 +0.3 N.D.
auxotrophy Dropout Complete +0.2 -0.3 -0.4 +0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.1 -0.5 +0.3 N.D.
growth on ypd YPD +0.4 +0.5 -0.3 -0.5 N.D.
auxotrophy YNB Glucose +0.1 -0.2 -0.3 +0.6 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 +0.1 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.0 +0.2 +0.3 N.D.
growth on oleic acid YNB Glucose -0.1 +0.5 +0.0 +0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.0 +0.5 -0.3 +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.1 -0.1 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.6 +0.9 -0.6 +0.4 N.D.
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