Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8708 and 8709 are separated by 188 nucleotides8709 and 8710 are separated by 268 nucleotides8710 and 8711 are separated by 59 nucleotides8711 and 8712 are separated by 167 nucleotides mRNA_340: 8708 - KOG1271 Methyltransferases, at 799,973 to 801,137 8708 mRNA_341: 8709 - K00860 cysC adenylylsulfate kinase, at 801,326 to 802,147 8709 mRNA_342: 8710 - SUPERFAMILY--SSF54285, at 802,416 to 802,761 8710 mRNA_343: 8711 - HMMPfam-Domain of unknown function (DUF4078)-PF13300, at 802,821 to 804,087 8711 mRNA_344: 8712 - K00914 PIK3C3, VPS34 phosphatidylinositol 3-kinase, at 804,255 to 807,496 8712
Group Condition mRNA_340 mRNA_341 mRNA_342 mRNA_343 mRNA_344
8708 8709 8710 8711 8712
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.0 -0.4 -3.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -2.4 +0.1 -2.1 N.D.
auxotrophy YNB Glucose -0.4 -3.0 +0.0 +0.3 N.D.
growth on ricinoleic acid YNB Glucose -0.1 -2.8 +0.2 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 -3.0 +0.0 +0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -2.0 -0.1 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 -2.9 +0.0 -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.0 +0.0 -1.8 N.D.
growth on oleic acid YNB Glucose +0.3 -2.3 +0.2 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.6 -0.7 -0.1 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -1.6 -0.1 -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.1 -0.6 +0.3 N.D.
growth on ypd YPD +0.3 +0.1 -0.5 -0.5 N.D.
auxotrophy Dropout Complete -0.3 -0.1 +0.0 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.0 -0.1 -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.0 -0.2 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.1 +0.2 +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 -0.4 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.4 -0.3 +0.3 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 -0.5 -0.0 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +1.1 -0.0 +0.6 N.D.
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