Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8709 and 8710 are separated by 268 nucleotides8710 and 8711 are separated by 59 nucleotides8711 and 8712 are separated by 167 nucleotides8712 and 8713 are separated by 336 nucleotides mRNA_341: 8709 - K00860 cysC adenylylsulfate kinase, at 801,326 to 802,147 8709 mRNA_342: 8710 - SUPERFAMILY--SSF54285, at 802,416 to 802,761 8710 mRNA_343: 8711 - HMMPfam-Domain of unknown function (DUF4078)-PF13300, at 802,821 to 804,087 8711 mRNA_344: 8712 - K00914 PIK3C3, VPS34 phosphatidylinositol 3-kinase, at 804,255 to 807,496 8712 mRNA_345: 8713 - HMMPfam-Mis12-Mtw1 protein family-PF08202, at 807,833 to 810,278 8713
Group Condition mRNA_341 mRNA_342 mRNA_343 mRNA_344 mRNA_345
8709 8710 8711 8712 8713
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.4 -3.0 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -2.4 +0.1 -2.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.9 +0.0 -0.1 N.D. N.D.
auxotrophy YNB Glucose -3.0 +0.0 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Glucose -2.8 +0.2 -0.0 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -2.0 -0.1 -0.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -3.0 +0.0 +0.6 N.D. N.D.
growth on oleic acid YNB Glucose -2.3 +0.2 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.6 -0.1 -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.0 -1.8 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.7 -0.1 -0.3 N.D. N.D.
growth on ypd YPD +0.1 -0.5 -0.5 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.6 +0.3 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 -0.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.2 +0.1 N.D. N.D.
auxotrophy Dropout Complete -0.1 +0.0 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.4 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.0 +0.5 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.3 +0.3 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.1 -0.0 +0.6 N.D. N.D.
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