Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8711 and 8712 are separated by 167 nucleotides8712 and 8713 are separated by 336 nucleotides8713 and 8714 are separated by 656 nucleotides8714 and 8715 are separated by 189 nucleotides mRNA_343: 8711 - HMMPfam-Domain of unknown function (DUF4078)-PF13300, at 802,821 to 804,087 8711 mRNA_344: 8712 - K00914 PIK3C3, VPS34 phosphatidylinositol 3-kinase, at 804,255 to 807,496 8712 mRNA_345: 8713 - HMMPfam-Mis12-Mtw1 protein family-PF08202, at 807,833 to 810,278 8713 mRNA_346: 8714 - KOG3113 Uncharacterized conserved protein, at 810,935 to 812,073 8714 mRNA_347: 8715 - K01358 clpP, CLPP ATP-dependent Clp protease, protease subunit, at 812,263 to 813,594 8715
Group Condition mRNA_343 mRNA_344 mRNA_345 mRNA_346 mRNA_347
8711 8712 8713 8714 8715
facs on lipid accumulation Sorted Low BODIPY Gate -3.0 N.D. N.D. +0.7 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -2.1 N.D. N.D. -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -1.8 N.D. N.D. +0.5 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. -0.5 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.5 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. N.D. +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. -0.2 +0.0
auxotrophy Dropout Complete -0.1 N.D. N.D. +0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. N.D. -0.7 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. N.D. +0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. N.D. -0.4 -0.3
growth on ricinoleic acid YNB Glucose -0.0 N.D. N.D. +0.1 -0.1
growth on ypd YPD -0.5 N.D. N.D. +0.4 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. N.D. -0.5 +0.2
auxotrophy YNB Glucose +0.3 N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.1 +0.6
growth on oleic acid YNB Oleic Acid +0.2 N.D. N.D. +0.4 +0.1
growth on oleic acid YNB Glucose +0.1 N.D. N.D. +0.7 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. N.D. +0.8 +0.5
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