Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8746 and 8747 are separated by 163 nucleotides8747 and 8748 are separated by 161 nucleotides8748 and 8749 are separated by 170 nucleotides8749 and 8750 are separated by 250 nucleotides mRNA_378: 8746 - K05012 CLCN3_4_5 chloride channel 3/4/5, at 879,759 to 883,039 8746 mRNA_379: 8747 - HMMPfam-Inositolphosphorylceramide synthase subunit Kei1-PF08552, at 883,203 to 884,818 8747 mRNA_380: 8748 - K15322 TSEN2 tRNA-splicing endonuclease subunit Sen2, at 884,980 to 886,576 8748 mRNA_381: 8749 - K10415 DYNC1I, DNCI dynein intermediate chain, cytosolic, at 886,747 to 889,332 8749 mRNA_382: 8750 - Hypothetical Protein, at 889,583 to 890,268 8750
Group Condition mRNA_378 mRNA_379 mRNA_380 mRNA_381 mRNA_382
8746 8747 8748 8749 8750
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. N.D. -0.8
auxotrophy YNB Glucose N.D. +0.1 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 N.D. N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 N.D. N.D. -0.7
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. N.D. -0.3
auxotrophy Dropout Complete N.D. +0.4 N.D. N.D. -0.5
growth on oleic acid YNB Glucose N.D. +0.3 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 N.D. N.D. +0.4
growth on oleic acid YNB Oleic Acid N.D. +0.3 N.D. N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 N.D. N.D. +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.4 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 N.D. N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 N.D. N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 N.D. N.D. +0.3
growth on ypd YPD N.D. +0.7 N.D. N.D. -0.0
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