Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8779 and 8780 are separated by 688 nucleotides8780 and 8781 are separated by 96 nucleotides8781 and 8782 are separated by 369 nucleotides8782 and 8783 are separated by 373 nucleotides mRNA_411: 8779 - K12862 PLRG1, PRL1, PRP46 pleiotropic regulator 1, at 962,864 to 964,890 8779 mRNA_412: 8780 - Hypothetical Protein, at 965,579 to 965,773 8780 mRNA_413: 8781 - K00227 SC5DL, ERG3 Delta7-sterol 5-desaturase, at 965,870 to 967,292 8781 mRNA_414: 8782 - KOG4170 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/Peroxisomal 3-ketoacyl-CoA-thiolase, sterol-binding domain and related enzymes, at 967,662 to 968,667 8782 mRNA_415: 8783 - K03511 POLK DNA polymerase kappa, at 969,041 to 971,775 8783
Group Condition mRNA_411 mRNA_412 mRNA_413 mRNA_414 mRNA_415
8779 8780 8781 8782 8783
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.6 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.4 -0.2
auxotrophy Dropout Complete N.D. N.D. N.D. -0.3 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.4 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.4 -0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.2 -0.2
auxotrophy YNB Glucose N.D. N.D. N.D. -0.2 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. +0.2 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.3 +0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.2 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.2 -0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.0 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. +0.3 -0.0
growth on ypd YPD N.D. N.D. N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.4 +0.1
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