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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8780 and 8781 are separated by 96 nucleotides
8781 and 8782 are separated by 369 nucleotides
8782 and 8783 are separated by 373 nucleotides
8783 and 8784 are separated by 270 nucleotides
mRNA_412: 8780 - Hypothetical Protein, at 965,579 to 965,773
8780
mRNA_413: 8781 - K00227 SC5DL, ERG3 Delta7-sterol 5-desaturase, at 965,870 to 967,292
8781
mRNA_414: 8782 - KOG4170 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/Peroxisomal 3-ketoacyl-CoA-thiolase, sterol-binding domain and related enzymes, at 967,662 to 968,667
8782
mRNA_415: 8783 - K03511 POLK DNA polymerase kappa, at 969,041 to 971,775
8783
mRNA_416: 8784 - Hypothetical Protein, at 972,046 to 973,465
8784
Group
Condition
mRNA
_412
mRNA
_413
mRNA
_414
mRNA
_415
mRNA
_416
8780
8781
8782
8783
8784
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
N.D.
-0.6
-0.1
-0.3
auxotrophy
YNB Glucose 75mM Methionine
N.D.
N.D.
-0.4
-0.2
-0.2
auxotrophy
YNB Glucose 75mM Arginine
N.D.
N.D.
-0.4
-0.0
-0.2
auxotrophy
Dropout Complete
N.D.
N.D.
-0.3
-0.2
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
N.D.
+0.2
-0.4
-0.1
auxotrophy
YNB Glucose
N.D.
N.D.
-0.2
-0.0
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
N.D.
-0.4
-0.0
+0.0
growth on oleic acid
YNB Oleic Acid
N.D.
N.D.
-0.2
-0.2
+0.2
growth on oleic acid
YNB Glucose
N.D.
N.D.
-0.2
+0.0
+0.1
growth on ricinoleic acid
YNB Glucose
N.D.
N.D.
-0.1
+0.1
-0.1
ypd buoyancy
40 hrs Median Buoyancy
N.D.
N.D.
+0.1
+0.1
-0.1
ypd buoyancy
40 hrs Low Buoyancy
N.D.
N.D.
+0.0
+0.1
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
N.D.
+0.2
-0.1
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
N.D.
-0.3
+0.1
+0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
N.D.
+0.3
+0.1
-0.1
ypd buoyancy
40 hrs High Buoyancy
N.D.
N.D.
+0.2
+0.2
-0.0
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
N.D.
+0.3
-0.0
+0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
N.D.
+0.3
+0.1
+0.0
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
N.D.
+0.2
+0.0
+0.2
growth on ypd
YPD
N.D.
N.D.
+0.2
+0.1
+0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
N.D.
+0.4
+0.1
+0.3
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