Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8780 and 8781 are separated by 96 nucleotides8781 and 8782 are separated by 369 nucleotides8782 and 8783 are separated by 373 nucleotides8783 and 8784 are separated by 270 nucleotides mRNA_412: 8780 - Hypothetical Protein, at 965,579 to 965,773 8780 mRNA_413: 8781 - K00227 SC5DL, ERG3 Delta7-sterol 5-desaturase, at 965,870 to 967,292 8781 mRNA_414: 8782 - KOG4170 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/Peroxisomal 3-ketoacyl-CoA-thiolase, sterol-binding domain and related enzymes, at 967,662 to 968,667 8782 mRNA_415: 8783 - K03511 POLK DNA polymerase kappa, at 969,041 to 971,775 8783 mRNA_416: 8784 - Hypothetical Protein, at 972,046 to 973,465 8784
Group Condition mRNA_412 mRNA_413 mRNA_414 mRNA_415 mRNA_416
8780 8781 8782 8783 8784
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.6 -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.4 -0.0 -0.2
auxotrophy Dropout Complete N.D. N.D. -0.3 -0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.2 -0.4 -0.1
auxotrophy YNB Glucose N.D. N.D. -0.2 -0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.4 -0.0 +0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 -0.2 +0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.2 +0.0 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.0 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.3 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.3 +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 +0.2 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.3 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.3 +0.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 +0.0 +0.2
growth on ypd YPD N.D. N.D. +0.2 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 +0.1 +0.3
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