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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8781 and 8782 are separated by 369 nucleotides
8782 and 8783 are separated by 373 nucleotides
8783 and 8784 are separated by 270 nucleotides
8784 and 8785 are separated by 124 nucleotides
mRNA_413: 8781 - K00227 SC5DL, ERG3 Delta7-sterol 5-desaturase, at 965,870 to 967,292
8781
mRNA_414: 8782 - KOG4170 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/Peroxisomal 3-ketoacyl-CoA-thiolase, sterol-binding domain and related enzymes, at 967,662 to 968,667
8782
mRNA_415: 8783 - K03511 POLK DNA polymerase kappa, at 969,041 to 971,775
8783
mRNA_416: 8784 - Hypothetical Protein, at 972,046 to 973,465
8784
mRNA_417: 8785 - KOG4697 Integral membrane protein involved in transport between the late Golgi and endosome, at 973,590 to 974,560
8785
Group
Condition
mRNA
_413
mRNA
_414
mRNA
_415
mRNA
_416
mRNA
_417
8781
8782
8783
8784
8785
auxotrophy
YNB Glucose 75mM Methionine
N.D.
-0.4
-0.2
-0.2
-0.6
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
-0.6
-0.1
-0.3
-0.4
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
-0.4
-0.0
+0.0
-0.4
auxotrophy
Dropout Complete
N.D.
-0.3
-0.2
-0.1
-0.1
auxotrophy
YNB Glucose 75mM Arginine
N.D.
-0.4
-0.0
-0.2
-0.1
growth on ricinoleic acid
YNB Glucose
N.D.
-0.1
+0.1
-0.1
-0.7
auxotrophy
YNB Glucose
N.D.
-0.2
-0.0
-0.1
-0.3
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
+0.2
-0.4
-0.1
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
+0.2
-0.1
+0.1
-0.6
growth on oleic acid
YNB Glucose
N.D.
-0.2
+0.0
+0.1
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
+0.3
+0.1
+0.0
-0.6
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
+0.3
+0.1
-0.1
-0.4
growth on oleic acid
YNB Oleic Acid
N.D.
-0.2
-0.2
+0.2
+0.3
ypd buoyancy
40 hrs Median Buoyancy
N.D.
+0.1
+0.1
-0.1
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
+0.4
+0.1
+0.3
-0.5
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
+0.3
-0.0
+0.2
-0.1
ypd buoyancy
40 hrs Low Buoyancy
N.D.
+0.0
+0.1
+0.1
+0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
-0.3
+0.1
+0.4
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
+0.2
+0.0
+0.2
-0.1
ypd buoyancy
40 hrs High Buoyancy
N.D.
+0.2
+0.2
-0.0
+0.3
growth on ypd
YPD
N.D.
+0.2
+0.1
+0.4
+0.1
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