Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8782 and 8783 are separated by 373 nucleotides8783 and 8784 are separated by 270 nucleotides8784 and 8785 are separated by 124 nucleotides8785 and 8786 are separated by 1671 nucleotides mRNA_414: 8782 - KOG4170 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/Peroxisomal 3-ketoacyl-CoA-thiolase, sterol-binding domain and related enzymes, at 967,662 to 968,667 8782 mRNA_415: 8783 - K03511 POLK DNA polymerase kappa, at 969,041 to 971,775 8783 mRNA_416: 8784 - Hypothetical Protein, at 972,046 to 973,465 8784 mRNA_417: 8785 - KOG4697 Integral membrane protein involved in transport between the late Golgi and endosome, at 973,590 to 974,560 8785 mRNA_418: 8786 - KOG2308 Phosphatidic acid-preferring phospholipase A1, contains DDHD domain, at 976,232 to 979,073 8786
Group Condition mRNA_414 mRNA_415 mRNA_416 mRNA_417 mRNA_418
8782 8783 8784 8785 8786
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.2 -0.2 -0.6 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 -0.1 -0.3 -0.4 +0.0
auxotrophy Dropout Complete -0.3 -0.2 -0.1 -0.1 -0.0
auxotrophy YNB Glucose -0.2 -0.0 -0.1 -0.3 -0.1
growth on ricinoleic acid YNB Glucose -0.1 +0.1 -0.1 -0.7 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.0 -0.2 -0.1 +0.1
growth on oleic acid YNB Glucose -0.2 +0.0 +0.1 -0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.0 +0.0 -0.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.4 -0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.1 +0.1 -0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.1 -0.1 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.1 +0.0 -0.6 +0.1
growth on oleic acid YNB Oleic Acid -0.2 -0.2 +0.2 +0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 -0.1 +0.1 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 +0.2 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 +0.3 -0.5 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.0 +0.2 -0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.1 +0.4 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 +0.1 +0.2 +0.1
growth on ypd YPD +0.2 +0.1 +0.4 +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.2 -0.0 +0.3 +0.0
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