Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8798 and 8799 are separated by 394 nucleotides8799 and 8800 are separated by 248 nucleotides8800 and 8801 are separated by 298 nucleotides8801 and 8802 are separated by 508 nucleotides mRNA_430: 8798 - K13704 ABHD12 abhydrolase domain-containing protein 12, at 998,852 to 1,000,348 8798 mRNA_431: 8799 - HMMPfam-SH3-binding, glutamic acid-rich protein-PF04908,ProSiteProfiles-Glutaredoxin domain profile.-PS51354,SUPERFAMILY--SSF52833, at 1,000,743 to 1,002,192 8799 mRNA_432: 8800 - KOG4546 Peroxisomal biogenesis protein (peroxin 16), at 1,002,441 to 1,004,033 8800 mRNA_433: 8801 - K03247 EIF3H translation initiation factor 3 subunit H, at 1,004,332 to 1,006,168 8801 mRNA_434: 8802 - K08738 CYC cytochrome c, at 1,006,677 to 1,007,742 8802
Group Condition mRNA_430 mRNA_431 mRNA_432 mRNA_433 mRNA_434
8798 8799 8800 8801 8802
auxotrophy Dropout Complete -0.5 -0.6 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.9 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.6 -0.0 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.8 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.6 +0.0 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.2 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.5 N.D. N.D. N.D.
auxotrophy YNB Glucose -0.1 -0.3 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.4 N.D. N.D. N.D.
growth on oleic acid YNB Glucose -0.4 +0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.3 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 -0.6 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.6 -0.2 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.6 -0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 +0.2 N.D. N.D. N.D.
growth on ypd YPD +0.8 +0.3 N.D. N.D. N.D.
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