Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8809 and 8810 are separated by 279 nucleotides8810 and 8811 are separated by 225 nucleotides8811 and 8812 are separated by 335 nucleotides8812 and 8813 are separated by 279 nucleotides mRNA_441: 8809 - K13989 DERL2_3 Derlin-2/3, at 1,022,824 to 1,024,299 8809 mRNA_442: 8810 - K11835 USP4_11_15, UBP12 ubiquitin carboxyl-terminal hydrolase 4/11/15, at 1,024,579 to 1,029,147 8810 mRNA_443: 8811 - KOG2742 Predicted oxidoreductase, at 1,029,373 to 1,030,485 8811 mRNA_444: 8812 - HMMPfam-Transcription factor IIIC subunit delta N-term-PF12657,HMMPfam-Putative zinc-finger of transcription factor IIIC complex-PF12660,SUPERFAMILY--SSF50978, at 1,030,821 to 1,034,414 8812 mRNA_445: 8813 - K03768 PPIB, ppiB peptidyl-prolyl cis-trans isomerase B (cyclophilin B), at 1,034,694 to 1,036,039 8813
Group Condition mRNA_441 mRNA_442 mRNA_443 mRNA_444 mRNA_445
8809 8810 8811 8812 8813
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 -1.0 +0.1 +0.4 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.7 +0.0 -0.1 -0.3 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.0 -0.5 -0.1 -0.4
growth on oleic acid YNB Glucose -0.8 +0.0 +0.3 -0.2 +0.0
growth on ricinoleic acid YNB Glucose -0.8 +0.2 +1.0 +0.3 -0.6
auxotrophy Dropout Complete -0.6 +0.2 +1.0 -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.4 -0.0 +0.4 -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.9 -0.7 +0.4 -0.3 -0.0
growth on oleic acid YNB Oleic Acid -0.2 +0.1 +0.1 +0.2 +0.1
auxotrophy YNB Glucose 75mM Methionine -1.2 +0.3 +1.4 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.0 +0.6 -0.3 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.1 +0.3 -0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 -0.3 +0.2 +0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.0 +0.7 +0.7 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.5 -0.3 +0.0 +0.6
auxotrophy YNB Glucose -0.7 +0.3 +1.1 +0.5 -0.3
auxotrophy YNB Glucose 75mM Arginine -1.0 +0.3 +1.5 +0.5 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 +0.2 +1.5 +0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 -0.4 +0.2 +0.4 +0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.0 +0.5 +0.3 +0.5
growth on ypd YPD +1.9 +0.2 -0.2 +0.5 +0.6
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