Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8812 and 8813 are separated by 279 nucleotides8813 and 8814 are separated by 856 nucleotides8814 and 8815 are separated by 1322 nucleotides8815 and 8816 are separated by 618 nucleotides mRNA_444: 8812 - HMMPfam-Transcription factor IIIC subunit delta N-term-PF12657,HMMPfam-Putative zinc-finger of transcription factor IIIC complex-PF12660,SUPERFAMILY--SSF50978, at 1,030,821 to 1,034,414 8812 mRNA_445: 8813 - K03768 PPIB, ppiB peptidyl-prolyl cis-trans isomerase B (cyclophilin B), at 1,034,694 to 1,036,039 8813 mRNA_446: 8814 - K00254 DHODH, pyrD dihydroorotate dehydrogenase, at 1,036,896 to 1,038,649 8814 mRNA_447: 8815 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 1,039,972 to 1,042,565 8815 mRNA_448: 8816 - K20221 IPO4, RANBP4 importin-4, at 1,043,184 to 1,046,908 8816
Group Condition mRNA_444 mRNA_445 mRNA_446 mRNA_447 mRNA_448
8812 8813 8814 8815 8816
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.4 N.D. -1.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.0 N.D. -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 N.D. -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.3 N.D. -0.2 N.D.
auxotrophy Dropout Complete -0.1 -0.4 N.D. +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose -0.2 +0.0 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.3 -0.6 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.2 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.6 N.D. -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.3 N.D. +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.2 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.5 N.D. -0.1 N.D.
auxotrophy YNB Glucose +0.5 -0.3 N.D. +0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.2 N.D. +0.5 N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.1 N.D. +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 -0.4 N.D. +0.7 N.D.
growth on ypd YPD +0.5 +0.6 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.9 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +0.1 N.D. +1.1 N.D.
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