Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8813 and 8814 are separated by 856 nucleotides8814 and 8815 are separated by 1322 nucleotides8815 and 8816 are separated by 618 nucleotides8816 and 8817 are separated by 278 nucleotides mRNA_445: 8813 - K03768 PPIB, ppiB peptidyl-prolyl cis-trans isomerase B (cyclophilin B), at 1,034,694 to 1,036,039 8813 mRNA_446: 8814 - K00254 DHODH, pyrD dihydroorotate dehydrogenase, at 1,036,896 to 1,038,649 8814 mRNA_447: 8815 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 1,039,972 to 1,042,565 8815 mRNA_448: 8816 - K20221 IPO4, RANBP4 importin-4, at 1,043,184 to 1,046,908 8816 mRNA_449: 8817 - HMMPfam-ESSS subunit of NADH-ubiquinone oxidoreductase (complex I)-PF10183, at 1,047,187 to 1,047,536 8817
Group Condition mRNA_445 mRNA_446 mRNA_447 mRNA_448 mRNA_449
8813 8814 8815 8816 8817
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. -1.1 N.D. -1.8
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.0 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. -0.4 N.D. -0.5
growth on ricinoleic acid YNB Glucose -0.6 N.D. +0.4 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. +0.7 N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. -0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.0 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. -0.2 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. +0.5 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.1 N.D. +0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.5 N.D. -0.6
auxotrophy Dropout Complete -0.4 N.D. +0.4 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.3 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.2 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.2 N.D. +0.4
auxotrophy YNB Glucose -0.3 N.D. +0.5 N.D. +0.0
growth on oleic acid YNB Glucose +0.0 N.D. +0.2 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +1.1 N.D. -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.1 N.D. +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 N.D. +0.0 N.D. +0.1
growth on ypd YPD +0.6 N.D. +0.0 N.D. +1.5
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