Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8815 and 8816 are separated by 618 nucleotides8816 and 8817 are separated by 278 nucleotides8817 and 8818 are separated by 616 nucleotides8818 and 8819 are separated by 567 nucleotides mRNA_447: 8815 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 1,039,972 to 1,042,565 8815 mRNA_448: 8816 - K20221 IPO4, RANBP4 importin-4, at 1,043,184 to 1,046,908 8816 mRNA_449: 8817 - HMMPfam-ESSS subunit of NADH-ubiquinone oxidoreductase (complex I)-PF10183, at 1,047,187 to 1,047,536 8817 mRNA_450: 8818 - K09525 DNAJC5 DnaJ homolog subfamily C member 5, at 1,048,153 to 1,050,379 8818 mRNA_451: 8819 - K00830 AGXT alanine-glyoxylate transaminase / serine-glyoxylate transaminase / serine-pyruvate transaminase, at 1,050,947 to 1,052,587 8819
Group Condition mRNA_447 mRNA_448 mRNA_449 mRNA_450 mRNA_451
8815 8816 8817 8818 8819
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 N.D. -1.8 +0.6 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.5 +0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.1 -0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. -0.5 -0.3 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 N.D. -0.5 -0.6 +0.3
growth on oleic acid YNB Oleic Acid +0.5 N.D. -0.6 -0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. -0.3 +0.5 -0.6
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.3 +0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. +0.2 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.2 +0.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -0.1 +0.2 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +1.1 N.D. -0.7 -0.5 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.4 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. +0.1 +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.2 -0.1 +0.4
growth on oleic acid YNB Glucose +0.2 N.D. +0.2 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.6 +0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.0 -0.2 +0.6
auxotrophy Dropout Complete +0.4 N.D. +0.1 -0.2 +0.4
auxotrophy YNB Glucose +0.5 N.D. +0.0 -0.1 +0.7
growth on ypd YPD +0.0 N.D. +1.5 +0.1 -0.3
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