Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8816 and 8817 are separated by 278 nucleotides8817 and 8818 are separated by 616 nucleotides8818 and 8819 are separated by 567 nucleotides8819 and 8820 are separated by 603 nucleotides mRNA_448: 8816 - K20221 IPO4, RANBP4 importin-4, at 1,043,184 to 1,046,908 8816 mRNA_449: 8817 - HMMPfam-ESSS subunit of NADH-ubiquinone oxidoreductase (complex I)-PF10183, at 1,047,187 to 1,047,536 8817 mRNA_450: 8818 - K09525 DNAJC5 DnaJ homolog subfamily C member 5, at 1,048,153 to 1,050,379 8818 mRNA_451: 8819 - K00830 AGXT alanine-glyoxylate transaminase / serine-glyoxylate transaminase / serine-pyruvate transaminase, at 1,050,947 to 1,052,587 8819 mRNA_452: 8820 - SUPERFAMILY--SSF52047, at 1,053,191 to 1,054,789 8820
Group Condition mRNA_448 mRNA_449 mRNA_450 mRNA_451 mRNA_452
8816 8817 8818 8819 8820
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.5 -0.6 +0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.5 -0.3 -0.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 +0.5 -0.6 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.7 -0.5 +0.3 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.8 +0.6 +0.6 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 +0.2 -0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.3 -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.6 -0.2 +0.2 +0.6
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 +0.1 +0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.1 +0.4 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.1 +0.2 -0.3
auxotrophy Dropout Complete N.D. +0.1 -0.2 +0.4 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 +0.4 +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 +0.2 +0.4 +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.0 -0.2 +0.6 +0.3
auxotrophy YNB Glucose N.D. +0.0 -0.1 +0.7 +0.1
growth on oleic acid YNB Glucose N.D. +0.2 -0.1 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 +0.1 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 -0.1 +0.2 +0.4
growth on ypd YPD N.D. +1.5 +0.1 -0.3 +0.1
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