Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8833 and 8834 are separated by 332 nucleotides8834 and 8835 are separated by 326 nucleotides8835 and 8836 are separated by 81 nucleotides8836 and 8837 are separated by 846 nucleotides mRNA_465: 8833 - K10858 PMS2 DNA mismatch repair protein PMS2, at 1,079,162 to 1,083,033 8833 mRNA_466: 8834 - Hypothetical Protein, at 1,083,366 to 1,084,523 8834 mRNA_467: 8835 - K07748 E1.1.1.170, NSDHL, ERG26 sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating), at 1,084,850 to 1,086,342 8835 mRNA_468: 8836 - Hypothetical Protein, at 1,086,424 to 1,087,519 8836 mRNA_469: 8837 - KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein vp115/Granuphilin A, contains C2 domain, at 1,088,366 to 1,093,699 8837
Group Condition mRNA_465 mRNA_466 mRNA_467 mRNA_468 mRNA_469
8833 8834 8835 8836 8837
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.3 N.D. +0.2 -1.5
auxotrophy YNB Glucose 75mM Arginine -0.5 -0.2 N.D. +0.3 -1.1
auxotrophy YNB Glucose -0.2 -0.0 N.D. +0.1 -1.4
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.2 N.D. +0.0 -1.5
auxotrophy Dropout Complete -0.2 -0.0 N.D. +0.2 -1.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.1 N.D. +0.5 -1.6
growth on ricinoleic acid YNB Glucose -0.3 +0.0 N.D. +0.3 -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.2 N.D. -0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 N.D. -0.2 -0.7
growth on oleic acid YNB Oleic Acid -0.2 -0.2 N.D. +0.5 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. -0.3 -0.3
growth on ypd YPD +0.0 +0.0 N.D. +0.3 -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.1 N.D. -0.5 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.1 N.D. +0.4 -0.5
growth on oleic acid YNB Glucose -0.1 -0.2 N.D. +0.4 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 N.D. +0.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.5 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 N.D. +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.3 N.D. -0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 N.D. +0.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.6 N.D. +0.3 +0.9
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