Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8835 and 8836 are separated by 81 nucleotides8836 and 8837 are separated by 846 nucleotides8837 and 8838 are separated by 457 nucleotides8838 and 8839 are separated by 331 nucleotides mRNA_467: 8835 - K07748 E1.1.1.170, NSDHL, ERG26 sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating), at 1,084,850 to 1,086,342 8835 mRNA_468: 8836 - Hypothetical Protein, at 1,086,424 to 1,087,519 8836 mRNA_469: 8837 - KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein vp115/Granuphilin A, contains C2 domain, at 1,088,366 to 1,093,699 8837 mRNA_470: 8838 - KOG0714 Molecular chaperone (DnaJ superfamily), at 1,094,157 to 1,095,851 8838 mRNA_471: 8839 - Hypothetical Protein, at 1,096,183 to 1,097,842 8839
Group Condition mRNA_467 mRNA_468 mRNA_469 mRNA_470 mRNA_471
8835 8836 8837 8838 8839
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -1.5 -0.2 -0.6
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -1.5 +0.0 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 -1.6 -0.2 -0.2
auxotrophy Dropout Complete N.D. +0.2 -1.4 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -0.7 -0.3 +0.0
auxotrophy YNB Glucose N.D. +0.1 -1.4 +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 -1.1 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 -0.4 -0.4 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.3 -1.0 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 +0.0 -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 +0.1 -0.9 -0.0
growth on ypd YPD N.D. +0.3 -0.9 -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.3 -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.1 -0.2 +0.3
growth on oleic acid YNB Oleic Acid N.D. +0.5 -0.8 +0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.4 +0.1 -0.5 +0.1
growth on oleic acid YNB Glucose N.D. +0.4 -0.5 -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.1 -0.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -0.5 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 -0.3 +0.5 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 +0.9 +0.5 +0.2
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