Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8837 and 8838 are separated by 457 nucleotides8838 and 8839 are separated by 331 nucleotides8839 and 8840 are separated by 48 nucleotides8840 and 8841 are separated by 237 nucleotides mRNA_469: 8837 - KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein vp115/Granuphilin A, contains C2 domain, at 1,088,366 to 1,093,699 8837 mRNA_470: 8838 - KOG0714 Molecular chaperone (DnaJ superfamily), at 1,094,157 to 1,095,851 8838 mRNA_471: 8839 - Hypothetical Protein, at 1,096,183 to 1,097,842 8839 mRNA_472: 8840 - KOG0987 DNA helicase PIF1/RRM3, at 1,097,891 to 1,099,308 8840 mRNA_473: 8841 - K15255 PIF1 ATP-dependent DNA helicase PIF1, at 1,099,546 to 1,101,190 8841
Group Condition mRNA_469 mRNA_470 mRNA_471 mRNA_472 mRNA_473
8837 8838 8839 8840 8841
growth on ricinoleic acid YNB Methylricinoleic Acid -1.5 -0.2 -0.6 N.D. -0.0
auxotrophy Dropout Complete -1.4 -0.1 -0.2 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine -1.5 +0.0 -0.3 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.6 -0.2 -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 -0.3 +0.0 N.D. -0.4
auxotrophy YNB Glucose -1.4 +0.1 +0.2 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.9 -0.0 N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine -1.1 +0.1 -0.3 N.D. +0.0
growth on ricinoleic acid YNB Glucose -1.0 -0.1 -0.1 N.D. +0.2
growth on ypd YPD -0.9 -0.0 +0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.4 +0.2 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.5 +0.1 N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.8 +0.2 +0.2 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 +0.4 +0.1 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.0 -0.2 N.D. +0.2
growth on oleic acid YNB Glucose -0.5 -0.0 +0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.3 +0.2 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.0 +0.1 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.2 +0.3 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.5 +0.7 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 +0.5 +0.2 N.D. -0.0
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