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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8841 and 8842 are separated by 714 nucleotides
8842 and 8843 are separated by 366 nucleotides
8843 and 8844 are separated by 519 nucleotides
8844 and 8845 are separated by 1530 nucleotides
mRNA_473: 8841 - K15255 PIF1 ATP-dependent DNA helicase PIF1, at 1,099,546 to 1,101,190
8841
mRNA_474: 8842 - Hypothetical Protein, at 1,101,905 to 1,103,305
8842
mRNA_475: 8843 - K15153 MED31, SOH1 mediator of RNA polymerase II transcription subunit 31, at 1,103,672 to 1,105,927
8843
mRNA_476: 8844 - K02951 RP-S12e, RPS12 small subunit ribosomal protein S12e, at 1,106,447 to 1,107,237
8844
mRNA_477: 8845 - K10256 FAD2 omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase), at 1,108,768 to 1,110,470
8845
Group
Condition
mRNA
_473
mRNA
_474
mRNA
_475
mRNA
_476
mRNA
_477
8841
8842
8843
8844
8845
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.4
-0.7
+0.0
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.6
-0.3
-0.2
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.5
-0.3
+0.0
N.D.
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
-0.3
-0.2
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.0
-0.4
-0.1
N.D.
N.D.
auxotrophy
Dropout Complete
-0.4
+0.1
-0.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.1
-0.4
+0.2
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-0.0
-0.2
-0.0
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
+0.0
-0.3
+0.1
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
-0.0
+0.1
-0.2
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
+0.3
-0.2
N.D.
N.D.
growth on oleic acid
YNB Glucose
+0.0
+0.1
-0.1
N.D.
N.D.
growth on ypd
YPD
+0.2
-0.1
-0.1
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
+0.0
+0.1
+0.0
N.D.
N.D.
auxotrophy
YNB Glucose
-0.2
+0.2
+0.1
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
+0.2
+0.1
-0.1
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
+0.2
+0.1
+0.1
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.0
+0.3
+0.1
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
+0.2
+0.5
-0.2
N.D.
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
+0.8
-0.1
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
+0.5
+0.5
+0.0
N.D.
N.D.
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