Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8841 and 8842 are separated by 714 nucleotides8842 and 8843 are separated by 366 nucleotides8843 and 8844 are separated by 519 nucleotides8844 and 8845 are separated by 1530 nucleotides mRNA_473: 8841 - K15255 PIF1 ATP-dependent DNA helicase PIF1, at 1,099,546 to 1,101,190 8841 mRNA_474: 8842 - Hypothetical Protein, at 1,101,905 to 1,103,305 8842 mRNA_475: 8843 - K15153 MED31, SOH1 mediator of RNA polymerase II transcription subunit 31, at 1,103,672 to 1,105,927 8843 mRNA_476: 8844 - K02951 RP-S12e, RPS12 small subunit ribosomal protein S12e, at 1,106,447 to 1,107,237 8844 mRNA_477: 8845 - K10256 FAD2 omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase), at 1,108,768 to 1,110,470 8845
Group Condition mRNA_473 mRNA_474 mRNA_475 mRNA_476 mRNA_477
8841 8842 8843 8844 8845
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.7 +0.0 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.6 -0.3 -0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.3 +0.0 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.4 -0.1 N.D. N.D.
auxotrophy Dropout Complete -0.4 +0.1 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.4 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.2 -0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.0 -0.3 +0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.1 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.3 -0.2 N.D. N.D.
growth on oleic acid YNB Glucose +0.0 +0.1 -0.1 N.D. N.D.
growth on ypd YPD +0.2 -0.1 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.1 +0.0 N.D. N.D.
auxotrophy YNB Glucose -0.2 +0.2 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.1 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.2 +0.1 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.3 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.5 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.8 -0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 +0.5 +0.0 N.D. N.D.
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